BMRB Entry 34149
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34149
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Title: Solution NMR structure of human GATA2 C-terminal zinc finger domain
Deposition date: 2017-06-16 Original release date: 2019-02-01
Authors: Nurmohamed, S.; Broadhurst, R.; May, G.; Enver, T.
Citation: Nurmohamed, S.; Broadhurst, R.; May, G.; Enver, T.. "Solution NMR structure of human GATA2 C-terminal zinc finger domain" . ., .-..
Assembly members:
entity_1, polymer, 68 residues, 7700.792 Da.
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: MAHHHHHHSSGLEVLFQGPR
RAGTCCANCQTTTTTLWRRN
ANGDPVCNACGLYYKLHNVN
RPLTMKKE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 178 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 352 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 68 residues - 7700.792 Da.
| 1 | MET | ALA | HIS | HIS | HIS | HIS | HIS | HIS | SER | SER | ||||
| 2 | GLY | LEU | GLU | VAL | LEU | PHE | GLN | GLY | PRO | ARG | ||||
| 3 | ARG | ALA | GLY | THR | CYS | CYS | ALA | ASN | CYS | GLN | ||||
| 4 | THR | THR | THR | THR | THR | LEU | TRP | ARG | ARG | ASN | ||||
| 5 | ALA | ASN | GLY | ASP | PRO | VAL | CYS | ASN | ALA | CYS | ||||
| 6 | GLY | LEU | TYR | TYR | LYS | LEU | HIS | ASN | VAL | ASN | ||||
| 7 | ARG | PRO | LEU | THR | MET | LYS | LYS | GLU |
Entity 2, entity_2 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: GATA2 Zinc Finger 2, [U-99% 13C; U-99% 15N], 10 mg/L
sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 Pa; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA v2.3, Linge, O'Donoghue and Nilges - refinement, structure calculation
Analysis v2.4.1, CCPN - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker DRX500 500 MHz
- Bruker DRX800 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts