BMRB Entry 34185
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34185
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Title: 6th KOW domain of human hSpt5 PubMed: 30076330
Deposition date: 2017-10-16 Original release date: 2018-10-26
Authors: Hahn, L.; Schweimer, K.; Roesch, P.; Woehrl, B.
Citation: Zuber, P.; Hahn, L.; Reinl, A.; Schweimer, K.; Knauer, S.; Gottesman, M.; Rosch, P.; Wohrl, B.. "Structure and nucleic acid binding properties of KOW domains 4 and 6-7 of human transcription elongation factor DSIF." Sci. Rep. 8, 11660-11660 (2018).
Assembly members:
entity_1, polymer, 127 residues, 13857.621 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)
Entity Sequences (FASTA):
entity_1: GGYNPHTPGSGIEQNSSDWV
TTDIQVKVRDTYLDTQVVGQ
TGVIRSVTGGMCSVYLKDSE
KVVSISSEHLEPITPTKNNK
VKVILGEDREATGVLLSIDG
EDGIVRMDLDEQLKILNLRF
LGKLLEA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 516 |
| 15N chemical shifts | 124 |
| 1H chemical shifts | 817 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 127 residues - 13857.621 Da.
| 1 | GLY | GLY | TYR | ASN | PRO | HIS | THR | PRO | GLY | SER | ||||
| 2 | GLY | ILE | GLU | GLN | ASN | SER | SER | ASP | TRP | VAL | ||||
| 3 | THR | THR | ASP | ILE | GLN | VAL | LYS | VAL | ARG | ASP | ||||
| 4 | THR | TYR | LEU | ASP | THR | GLN | VAL | VAL | GLY | GLN | ||||
| 5 | THR | GLY | VAL | ILE | ARG | SER | VAL | THR | GLY | GLY | ||||
| 6 | MET | CYS | SER | VAL | TYR | LEU | LYS | ASP | SER | GLU | ||||
| 7 | LYS | VAL | VAL | SER | ILE | SER | SER | GLU | HIS | LEU | ||||
| 8 | GLU | PRO | ILE | THR | PRO | THR | LYS | ASN | ASN | LYS | ||||
| 9 | VAL | LYS | VAL | ILE | LEU | GLY | GLU | ASP | ARG | GLU | ||||
| 10 | ALA | THR | GLY | VAL | LEU | LEU | SER | ILE | ASP | GLY | ||||
| 11 | GLU | ASP | GLY | ILE | VAL | ARG | MET | ASP | LEU | ASP | ||||
| 12 | GLU | GLN | LEU | LYS | ILE | LEU | ASN | LEU | ARG | PHE | ||||
| 13 | LEU | GLY | LYS | LEU | LEU | GLU | ALA |
Samples:
sample_1: KOW6, [U-99% 13C; U-99% 15N], 0.44 mM; sodium phosphate 20 mM; sodium chloride 20 mM; DTT 0.5 mM
sample_conditions_1: ionic strength: 40 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculation
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Bruker AvanceIII 700 MHz
- Bruker AvanceIII 1000 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts