BMRB Entry 34220
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34220
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR Solution Structure of MINA-1(254-334) PubMed: 30728462
Deposition date: 2017-12-19 Original release date: 2019-01-28
Authors: Michel, E.; Allain, F.
Citation: Sendoel, Ataman; Subasic, Deni; Ducoli, Luca; Keller, Martin; Michel, Erich; Kohler, Ines; Singh, Kapil Dev; Zheng, Xue; Brummer, Anneke; Imig, Jochen; Kishore, Shivendra; Wu, Yibo; Kanitz, Alexander; Kaech, Andres; Mittal, Nitish; Matia-Gonzalez, Ana; Gerber, Andre; Zavolan, Mihaela; Aebersold, Ruedi; Hall, Jonathan; Allain, Frederic H-T; Hengartner, Michael. "MINA-1 and WAGO-4 are part of regulatory network coordinating germ cell death and RNAi in C. elegans" Cell Death Differ. ., .-. (2019).
Assembly members:
entity_1, polymer, 86 residues, 9634.081 Da.
Natural source: Common Name: C. elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3)
Entity Sequences (FASTA):
entity_1: SGSHMKTCVVEKIKQWIPTT
EVGKILGNRAAVKKHIERQF
NCVITVHTEVQSSFGATPVE
IVAQNKEQCQEARNAVMSLM
QSHQDK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 286 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 608 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 86 residues - 9634.081 Da.
| 1 | SER | GLY | SER | HIS | MET | LYS | THR | CYS | VAL | VAL | ||||
| 2 | GLU | LYS | ILE | LYS | GLN | TRP | ILE | PRO | THR | THR | ||||
| 3 | GLU | VAL | GLY | LYS | ILE | LEU | GLY | ASN | ARG | ALA | ||||
| 4 | ALA | VAL | LYS | LYS | HIS | ILE | GLU | ARG | GLN | PHE | ||||
| 5 | ASN | CYS | VAL | ILE | THR | VAL | HIS | THR | GLU | VAL | ||||
| 6 | GLN | SER | SER | PHE | GLY | ALA | THR | PRO | VAL | GLU | ||||
| 7 | ILE | VAL | ALA | GLN | ASN | LYS | GLU | GLN | CYS | GLN | ||||
| 8 | GLU | ALA | ARG | ASN | ALA | VAL | MET | SER | LEU | MET | ||||
| 9 | GLN | SER | HIS | GLN | ASP | LYS |
Samples:
sample_1: MINA-1(254-334), [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 2 mM; EDTA 0.5 mM
sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 293.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
ATNOS, Herrmann, Guntert and Wuthrich - peak picking
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
NMR spectrometers:
- Bruker Avance 900 MHz
- Bruker Avance 700 MHz
- Bruker Avance 600 MHz
- Bruker Avance 500 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts