BMRB Entry 34271
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34271
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Title: Structural insights into AapA1 toxin PubMed: 31476357
Deposition date: 2018-05-11 Original release date: 2019-09-06
Authors: Salgado, G.; Korkut, D.
Citation: Korkut, D.; Alves, I.; Vogel, A.; Chabas, S.; Sharma, C.; Martinez, D.; Loquet, A.; Salgado, G.; Darfeuille, F.. "Structural insights into the AapA1 toxin of Helicobacter pylori." Biochim. Biophys. Acta Gen. Subj. ., 129423-129423 (2019).
Assembly members:
entity_1, polymer, 30 residues, 3471.318 Da.
Natural source: Common Name: Helicobacter pylori Taxonomy ID: 210 Superkingdom: Bacteria Kingdom: not available Genus/species: Helicobacter pylori
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: MATKHGKNSWKTLYLKISFL
GCKVVVLLKR
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 31 |
| 1H chemical shifts | 222 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 30 residues - 3471.318 Da.
| 1 | MET | ALA | THR | LYS | HIS | GLY | LYS | ASN | SER | TRP | |
| 2 | LYS | THR | LEU | TYR | LEU | LYS | ILE | SER | PHE | LEU | |
| 3 | GLY | CYS | LYS | VAL | VAL | VAL | LEU | LEU | LYS | ARG |
Samples:
sample_1: AapA1_1 2 ± 0.1 mM; AapA1_2 0.2 ± 0.05 mM
sample_conditions_1: ionic strength: 50 mM; pH: 4.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
Sparky v3, Goddard - chemical shift assignment
CcpNmr Analysis, CCPN - chemical shift assignment
TopSpin, Bruker Biospin - collection
Sparky, Goddard - peak picking
NMR spectrometers:
- Bruker AVANCE III 800 MHz
- Bruker AVANCE III 700 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts