BMRB Entry 34337
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34337
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Pepducin UT-Pep2 a biased allosteric agonist of Urotensin-II receptor
Deposition date: 2018-12-15 Original release date: 2020-01-13
Authors: Carotenuto, A.; Hoang, T.; Nassour, H.; Martin, R.; Billard, E.; Myriam, L.; Novellino, E.; Tanny, J.; Fournier, A.; Hebert, T.; Chatenet, D.
Citation: Hoang, T.; Nassour, H.; Martin, R.; Billard, E.; Myriam, L.; Novellino, E.; Carotenuto, A.; Tanny, J.; Fournier, A.; Hebert, T.; Chatenet, D.. "Membrane-tethered peptides derived from intracellular loops 2 and 3 of the urotensin II receptor act as allosteric agonists promoting Gq/Gi activation, EGFR transactivation and fibroblast proliferation" . ., .-..
Assembly members:
entity_1, polymer, 14 residues, 1456.692 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: XRPLDTVQRPKGYX
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 95 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 14 residues - 1456.692 Da.
| 1 | ACE | ARG | PRO | LEU | ASP | THR | VAL | GLN | ARG | PRO | ||||
| 2 | LYS | GLY | TYR | NH2 |
Samples:
sample_1: peptide 2.0 mM; DPC, [U-99% 2H], 200.0 mM
sample_conditions_1: ionic strength: 0 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | anisotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | anisotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | anisotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation
XEASY, Bartels et al. - chemical shift assignment
NMR spectrometers:
- Varian INOVA 700 MHz