BMRB Entry 34428
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR34428
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Title: Mouse RBM20 RRM domain in complex with AUCUUA RNA PubMed: 32187365
Deposition date: 2019-08-29 Original release date: 2019-11-01
Authors: Mackereth, C.; Upadhyay, S.
Citation: Upadhyay, Santosh Kumar; Mackereth, Cameron. "Structural basis of UCUU RNA motif recognition by splicing factor RBM20" Nucleic Acids Res. 48, 4538-4550 (2020).
Assembly members:
entity_1, polymer, 6 residues, 1757.153 Da.
entity_2, polymer, 112 residues, 12571.588 Da.
Natural source: Common Name: not available Taxonomy ID: 32630 Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: XUCUUA
entity_2: GAMAQRKGAGRVVHICNLPE
GSCTENDVINLGLPFGKVTN
YILMKSTNQAFLEMAYTEAA
QAMVQYYQEKPAIINGEKLL
IRMSTRYKELQLKKPGKNVA
AIIQDIHSQRER
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 501 |
| 15N chemical shifts | 123 |
| 1H chemical shifts | 854 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 6 residues - 1757.153 Da.
| 1 | ADN | U | C | U | U | A |
Entity 2, entity_2 112 residues - 12571.588 Da.
| 1 | GLY | ALA | MET | ALA | GLN | ARG | LYS | GLY | ALA | GLY | ||||
| 2 | ARG | VAL | VAL | HIS | ILE | CYS | ASN | LEU | PRO | GLU | ||||
| 3 | GLY | SER | CYS | THR | GLU | ASN | ASP | VAL | ILE | ASN | ||||
| 4 | LEU | GLY | LEU | PRO | PHE | GLY | LYS | VAL | THR | ASN | ||||
| 5 | TYR | ILE | LEU | MET | LYS | SER | THR | ASN | GLN | ALA | ||||
| 6 | PHE | LEU | GLU | MET | ALA | TYR | THR | GLU | ALA | ALA | ||||
| 7 | GLN | ALA | MET | VAL | GLN | TYR | TYR | GLN | GLU | LYS | ||||
| 8 | PRO | ALA | ILE | ILE | ASN | GLY | GLU | LYS | LEU | LEU | ||||
| 9 | ILE | ARG | MET | SER | THR | ARG | TYR | LYS | GLU | LEU | ||||
| 10 | GLN | LEU | LYS | LYS | PRO | GLY | LYS | ASN | VAL | ALA | ||||
| 11 | ALA | ILE | ILE | GLN | ASP | ILE | HIS | SER | GLN | ARG | ||||
| 12 | GLU | ARG |
Samples:
sample_1: Mouse RBM20 RRM domain, [U-99% 13C; U-99% 15N], 400 uM; AUCUUA 480 uM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_2: Mouse RBM20 RRM domain, [U-99% 15N], 400 uM; AUCUUA 480 uM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_3: Mouse RBM20 RRM domain, [U-99% 13C; U-99% 15N], 500 uM; AUCUUA 600 uM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_4: Mouse RBM20 RRM domain, [U-99% 2H; U-99% 15N], 300 uM; AUCUUA 290 uM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_5: Mouse RBM20 RRM domain, [U-99% 13C], 300 uM; AUCUUA 360 uM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_6: Mouse RBM20 RRM domain, [U-10% 13C; U-99% 15N], 450 uM; AUCUUA 560 uM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_7: Mouse RBM20 RRM domain, [U-99% 13C; U-99% 15N], 500 uM; AUCUUA 600 uM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
sample_conditions_2: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_5 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC CT | sample_6 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_5 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_5 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_4 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_4 | isotropic | sample_conditions_1 |
| 2D x2-filtered 1H-1H NOESY | sample_7 | isotropic | sample_conditions_2 |
| 2D 1H-13C HSQC | sample_4 | isotropic | sample_conditions_1 |
| 2D x2-filtered 1H-1H NOESY | sample_5 | isotropic | sample_conditions_1 |
| 3D (H)C(CO)NH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_6 | isotropic | sample_conditions_1 |
Software:
TopSpin, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Sparky, Goddard - chemical shift assignment
ARIA, Linge, O'Donoghue and Nilges - structure calculation
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation
NMR spectrometers:
- Bruker Neo 700 MHz
- Bruker AVANCE III 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts