BMRB Entry 34443
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34443
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Title: Timeless couples G quadruplex detection with processing by DDX11 during DNA replication PubMed: 32705708
Deposition date: 2019-10-31 Original release date: 2020-06-26
Authors: Lerner Koch, L.; Holzer, S.; Kilkenny, M.; Murat, P.; Svikovic, S.; Schiavone, D.; Bittleston, A.; Maman, J.; Branzei, D.; Stott, K.; Pellegrini, L.; Sale, E.
Citation: Lerner, Leticia; Holzer, Sandro; Kilkenny, Mairi; Svikovic, Sasa; Murat, Pierre; Schiavone, Davide; Eldridge, Cara; Bittleston, Alice; Maman, Joseph; Branzei, Dana; Stott, Katherine; Pellegrini, Luca; Sale, Julian. "Timeless couples G quadruplex detection with processing by DDX11 during DNA replication" Embo J. ., e104185-e104185 (2020).
Assembly members:
entity_1, polymer, 141 residues, 16359.771 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: DPSRRAPTWSPEEEAHLREL
YLANKDVEGQDVVEAILAHL
NTVPRTRKQIIHHLVQMGLA
DSVKDFQRKGTHIVLWTGDQ
ELELQRLFEEFRDSDDVLGH
IMKNITAKRSRARIVDKLLA
LGLVAERRELYKKRQKKLAS
S
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 638 |
| 15N chemical shifts | 151 |
| 1H chemical shifts | 1041 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 141 residues - 16359.771 Da.
| 1 | ASP | PRO | SER | ARG | ARG | ALA | PRO | THR | TRP | SER | ||||
| 2 | PRO | GLU | GLU | GLU | ALA | HIS | LEU | ARG | GLU | LEU | ||||
| 3 | TYR | LEU | ALA | ASN | LYS | ASP | VAL | GLU | GLY | GLN | ||||
| 4 | ASP | VAL | VAL | GLU | ALA | ILE | LEU | ALA | HIS | LEU | ||||
| 5 | ASN | THR | VAL | PRO | ARG | THR | ARG | LYS | GLN | ILE | ||||
| 6 | ILE | HIS | HIS | LEU | VAL | GLN | MET | GLY | LEU | ALA | ||||
| 7 | ASP | SER | VAL | LYS | ASP | PHE | GLN | ARG | LYS | GLY | ||||
| 8 | THR | HIS | ILE | VAL | LEU | TRP | THR | GLY | ASP | GLN | ||||
| 9 | GLU | LEU | GLU | LEU | GLN | ARG | LEU | PHE | GLU | GLU | ||||
| 10 | PHE | ARG | ASP | SER | ASP | ASP | VAL | LEU | GLY | HIS | ||||
| 11 | ILE | MET | LYS | ASN | ILE | THR | ALA | LYS | ARG | SER | ||||
| 12 | ARG | ALA | ARG | ILE | VAL | ASP | LYS | LEU | LEU | ALA | ||||
| 13 | LEU | GLY | LEU | VAL | ALA | GLU | ARG | ARG | GLU | LEU | ||||
| 14 | TYR | LYS | LYS | ARG | GLN | LYS | LYS | LEU | ALA | SER | ||||
| 15 | SER |
Samples:
sample_1: TimDBD, [U-13C; U-15N], 0.5 mM; sodium phosphate, NA, 10 mM; potassium chloride, NA, 40 mM; EDTA, NA, 0.5 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
ARIA, Linge, O'Donoghue and Nilges - structure calculation
CcpNmr Analysis, CCPN - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AVANCE III 800 MHz
- Bruker AVANCE 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts