BMRB Entry 34466
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34466
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Title: Solution structure of U2AF2 RRM1,2 PubMed: 32188783
Deposition date: 2019-12-17 Original release date: 2020-04-28
Authors: Kang, H.; Sattler, M.
Citation: Kang, H.; Sanchez-Rico, C.; Ebersberger, S.; Sutandy, F.; Busch, A.; Welte, T.; Stehle, R.; Hipp, C.; Schulz, L.; Buchbender, A.; Zarnack, K.; Konig, J.; Sattler, M.. "An autoinhibitory intramolecular interaction proof-reads RNA recognition by the essential splicing factor U2AF2." Proc. Natl. Acad. Sci. U.S.A. 117, 7140-7149 (2020).
Assembly members:
entity_1, polymer, 205 residues, 22342.486 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: AMVGSQMTRQARRLYVGNIP
FGITEEAMMDFFNAQMRLGG
LTQAPGNPVLAVQINQDKNF
AFLEFRSVDETTQAMAFDGI
IFQGQSLKIRRPHDYQPLPG
MSENPSVYVPGVVSTVVPDS
AHKLFIGGLPNYLNDDQVKE
LLTSFGPLKAFNLVKDSATG
LSKGYAFCEYVDINVTDQAI
AGLNGMQLGDKKLLVQRASV
GAKNA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 655 |
| 15N chemical shifts | 215 |
| 1H chemical shifts | 1370 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 205 residues - 22342.486 Da.
| 1 | ALA | MET | VAL | GLY | SER | GLN | MET | THR | ARG | GLN | ||||
| 2 | ALA | ARG | ARG | LEU | TYR | VAL | GLY | ASN | ILE | PRO | ||||
| 3 | PHE | GLY | ILE | THR | GLU | GLU | ALA | MET | MET | ASP | ||||
| 4 | PHE | PHE | ASN | ALA | GLN | MET | ARG | LEU | GLY | GLY | ||||
| 5 | LEU | THR | GLN | ALA | PRO | GLY | ASN | PRO | VAL | LEU | ||||
| 6 | ALA | VAL | GLN | ILE | ASN | GLN | ASP | LYS | ASN | PHE | ||||
| 7 | ALA | PHE | LEU | GLU | PHE | ARG | SER | VAL | ASP | GLU | ||||
| 8 | THR | THR | GLN | ALA | MET | ALA | PHE | ASP | GLY | ILE | ||||
| 9 | ILE | PHE | GLN | GLY | GLN | SER | LEU | LYS | ILE | ARG | ||||
| 10 | ARG | PRO | HIS | ASP | TYR | GLN | PRO | LEU | PRO | GLY | ||||
| 11 | MET | SER | GLU | ASN | PRO | SER | VAL | TYR | VAL | PRO | ||||
| 12 | GLY | VAL | VAL | SER | THR | VAL | VAL | PRO | ASP | SER | ||||
| 13 | ALA | HIS | LYS | LEU | PHE | ILE | GLY | GLY | LEU | PRO | ||||
| 14 | ASN | TYR | LEU | ASN | ASP | ASP | GLN | VAL | LYS | GLU | ||||
| 15 | LEU | LEU | THR | SER | PHE | GLY | PRO | LEU | LYS | ALA | ||||
| 16 | PHE | ASN | LEU | VAL | LYS | ASP | SER | ALA | THR | GLY | ||||
| 17 | LEU | SER | LYS | GLY | TYR | ALA | PHE | CYS | GLU | TYR | ||||
| 18 | VAL | ASP | ILE | ASN | VAL | THR | ASP | GLN | ALA | ILE | ||||
| 19 | ALA | GLY | LEU | ASN | GLY | MET | GLN | LEU | GLY | ASP | ||||
| 20 | LYS | LYS | LEU | LEU | VAL | GLN | ARG | ALA | SER | VAL | ||||
| 21 | GLY | ALA | LYS | ASN | ALA |
Samples:
sample_1: U2AF2, [U-13C; U-15N], 0.5 mM; sodium phosphoate 20 mM; sodium chloride 50 mM; DTT 2 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
ARIA, Linge, O'Donoghue and Nilges - refinement
Sparky, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AVANCE III 600 MHz
- Bruker AVANCE III 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts