BMRB Entry 36026
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36026
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Title: Solution structure of human Gelsolin protein domain 1 at pH 5.0 PubMed: 28349924
Deposition date: 2016-10-26 Original release date: 2017-10-30
Authors: Fan, J.; Yang, D.
Citation: Fan, Jing-Song; Goh, Honzhen; Ding, Ke; Xue, Bo; Robinson, Robert; Yang, Daiwen. "Structural Basis for pH-mediated Regulation of F-actin Severing by Gelsolin Domain 1." Sci. Rep. 7, 45230-45230 (2017).
Assembly members:
entity_1, polymer, 133 residues, 14973.815 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: EHPEFLKAGKEPGLQIWRVE
KFDLVPVPTNLYGDFFTGDA
YVILKTVQLRNGNLQYDLHY
WLGNECSQDESGAAAIFTVQ
LDDYLNGRAVQHREVQGFES
ATFLGYFKSGLKYKKGGVAS
GFKHVVPNEVVVQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 114 |
| 15N chemical shifts | 127 |
| 1H chemical shifts | 742 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 133 residues - 14973.815 Da.
| 1 | GLU | HIS | PRO | GLU | PHE | LEU | LYS | ALA | GLY | LYS | ||||
| 2 | GLU | PRO | GLY | LEU | GLN | ILE | TRP | ARG | VAL | GLU | ||||
| 3 | LYS | PHE | ASP | LEU | VAL | PRO | VAL | PRO | THR | ASN | ||||
| 4 | LEU | TYR | GLY | ASP | PHE | PHE | THR | GLY | ASP | ALA | ||||
| 5 | TYR | VAL | ILE | LEU | LYS | THR | VAL | GLN | LEU | ARG | ||||
| 6 | ASN | GLY | ASN | LEU | GLN | TYR | ASP | LEU | HIS | TYR | ||||
| 7 | TRP | LEU | GLY | ASN | GLU | CYS | SER | GLN | ASP | GLU | ||||
| 8 | SER | GLY | ALA | ALA | ALA | ILE | PHE | THR | VAL | GLN | ||||
| 9 | LEU | ASP | ASP | TYR | LEU | ASN | GLY | ARG | ALA | VAL | ||||
| 10 | GLN | HIS | ARG | GLU | VAL | GLN | GLY | PHE | GLU | SER | ||||
| 11 | ALA | THR | PHE | LEU | GLY | TYR | PHE | LYS | SER | GLY | ||||
| 12 | LEU | LYS | TYR | LYS | LYS | GLY | GLY | VAL | ALA | SER | ||||
| 13 | GLY | PHE | LYS | HIS | VAL | VAL | PRO | ASN | GLU | VAL | ||||
| 14 | VAL | VAL | GLN |
Samples:
sample_1: gelsolin domain 1, [U-13C; U-15N], 0.65 ± 0.1 mM; Acetate 10 mM; H2O 90%; D2O, [U-2H], 10%
sample_conditions_1: ionic strength: 0 M; pH: 5.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 4D 13C_15N_edited NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.13, Goddard - data analysis, peak picking
XPLOR-NIH, C.D. Schwieters, J.J. Kuszewski - structure calculation
NMRPipe v8.7, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts