BMRB Entry 36034
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR36034
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Title: NMR structure of eIF3 36-163
Deposition date: 2016-11-21 Original release date: 2017-05-30
Authors: Nagata, T.; Obayashi, E.
Citation: Nagata, T.; Obayashi, E.. "NMR structure of eIF3 36-163" . ., .-..
Assembly members:
entity_1, polymer, 128 residues, 14598.317 Da.
Natural source: Common Name: Baker's yeast Taxonomy ID: 559292 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: SESELDQESDDSFFNESESE
SEADVDSDDSDAKPYGPDWF
KKSEFRKQGGGSNKFLKSSN
YDSSDEESDEEDGKKVVKSA
KEKLLDEMQDVYNKISQAEN
SDDWLTISNEFDLISRLLVR
AQQQNWGT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 429 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 601 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 128 residues - 14598.317 Da.
| 1 | SER | GLU | SER | GLU | LEU | ASP | GLN | GLU | SER | ASP | ||||
| 2 | ASP | SER | PHE | PHE | ASN | GLU | SER | GLU | SER | GLU | ||||
| 3 | SER | GLU | ALA | ASP | VAL | ASP | SER | ASP | ASP | SER | ||||
| 4 | ASP | ALA | LYS | PRO | TYR | GLY | PRO | ASP | TRP | PHE | ||||
| 5 | LYS | LYS | SER | GLU | PHE | ARG | LYS | GLN | GLY | GLY | ||||
| 6 | GLY | SER | ASN | LYS | PHE | LEU | LYS | SER | SER | ASN | ||||
| 7 | TYR | ASP | SER | SER | ASP | GLU | GLU | SER | ASP | GLU | ||||
| 8 | GLU | ASP | GLY | LYS | LYS | VAL | VAL | LYS | SER | ALA | ||||
| 9 | LYS | GLU | LYS | LEU | LEU | ASP | GLU | MET | GLN | ASP | ||||
| 10 | VAL | TYR | ASN | LYS | ILE | SER | GLN | ALA | GLU | ASN | ||||
| 11 | SER | ASP | ASP | TRP | LEU | THR | ILE | SER | ASN | GLU | ||||
| 12 | PHE | ASP | LEU | ILE | SER | ARG | LEU | LEU | VAL | ARG | ||||
| 13 | ALA | GLN | GLN | GLN | ASN | TRP | GLY | THR |
Samples:
sample_1: eIF3c 36-163, [U-100% 13C; U-100% 15N], 0.4 mM; DTT 1 mM; sodium chloride 150 mM; sodium phosphate 20 mM; H2O 95%; D2O, [U-2H], 5%
sample_conditions_1: ionic strength: 170 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
AMBER v9, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation
Kujira v0.98, Naohiro Kobayashi - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - peak picking
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts