BMRB Entry 36075

Name: The PHD finger of human Kiaa1045 protein
Published: 2018-04-30

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PDB ID: 5xht
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR36075
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: The PHD finger of human Kiaa1045 protein PubMed: 29430827

Deposition date: 2017-04-24 Original release date: 2018-04-30

Authors: Miyamoto, K.

Citation: Miyamoto, Kazuhide; Yamashita, Ayumi; Saito, Kazuki. "Solution structure of the PHD finger from the human KIAA1045 protein" Protein Sci. 27, 987-992 (2018).

Assembly members:
PHD finger protein 24, polymer, 60 residues, 6858.710 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
PHD finger protein 24: EMCDVCEVWTAESLFPCRVC TRVFHDGCLRRMGYIQGDSA AEVTEMAHTETGWSCHYCDN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	246
15N chemical shifts	55
1H chemical shifts	371

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_ZN_1	2
3	entity_ZN_2	2

Entities:

Entity 1, entity_1 60 residues - 6858.710 Da.

1	GLU	MET	CYS	ASP	VAL	CYS	GLU	VAL	TRP	THR
2	ALA	GLU	SER	LEU	PHE	PRO	CYS	ARG	VAL	CYS
3	THR	ARG	VAL	PHE	HIS	ASP	GLY	CYS	LEU	ARG
4	ARG	MET	GLY	TYR	ILE	GLN	GLY	ASP	SER	ALA
5	ALA	GLU	VAL	THR	GLU	MET	ALA	HIS	THR	GLU
6	THR	GLY	TRP	SER	CYS	HIS	TYR	CYS	ASP	ASN

Entity 2, entity_ZN_1 - Zn 2 - 65.409 Da.

1

ZN

Samples:

sample_1: PHD finger, [U-13C; U-15N], 1 mM; Tris-HCl 20 mM; NaCl 50 mM; dithiothreitol 1 mM; ZnCl2 50 uM; H2O 90%; D2O, U-2H, 10%

sample_conditions_1: ionic strength: 70 mM; pH: 6.9; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - refinement

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

1	GLU	MET	CYS	ASP	VAL	CYS	GLU	VAL	TRP	THR
2	ALA	GLU	SER	LEU	PHE	PRO	CYS	ARG	VAL	CYS
3	THR	ARG	VAL	PHE	HIS	ASP	GLY	CYS	LEU	ARG
4	ARG	MET	GLY	TYR	ILE	GLN	GLY	ASP	SER	ALA
5	ALA	GLU	VAL	THR	GLU	MET	ALA	HIS	THR	GLU
6	THR	GLY	TRP	SER	CYS	HIS	TYR	CYS	ASP	ASN

1	GLU	MET	CYS	ASP	VAL	CYS	GLU	VAL	TRP	THR
2	ALA	GLU	SER	LEU	PHE	PRO	CYS	ARG	VAL	CYS
3	THR	ARG	VAL	PHE	HIS	ASP	GLY	CYS	LEU	ARG
4	ARG	MET	GLY	TYR	ILE	GLN	GLY	ASP	SER	ALA
5	ALA	GLU	VAL	THR	GLU	MET	ALA	HIS	THR	GLU
6	THR	GLY	TRP	SER	CYS	HIS	TYR	CYS	ASP	ASN

1	GLU	MET	CYS	ASP	VAL	CYS	GLU	VAL	TRP	THR
2	ALA	GLU	SER	LEU	PHE	PRO	CYS	ARG	VAL	CYS
3	THR	ARG	VAL	PHE	HIS	ASP	GLY	CYS	LEU	ARG
4	ARG	MET	GLY	TYR	ILE	GLN	GLY	ASP	SER	ALA
5	ALA	GLU	VAL	THR	GLU	MET	ALA	HIS	THR	GLU
6	THR	GLY	TRP	SER	CYS	HIS	TYR	CYS	ASP	ASN