BMRB Entry 36179
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR36179
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Title: Solution structure of centrin4 from Trypanosoma brucei PubMed: 30389845
Deposition date: 2018-04-15 Original release date: 2019-03-11
Authors: Shan, F.; Tu, X.
Citation: Shan, Fangzhen; Ye, Kaiqin; Zhang, Jiahai; Liao, Shanhui; Zhang, Xuecheng; Xu, Chao; Tu, Xiaoming. "Solution structure of TbCentrin4 from" Biochem. J. 475, 3763-3778 (2018).
Assembly members:
Centrin, putative, polymer, 149 residues, 16570.627 Da.
Natural source: Common Name: Trypanosoma brucei Taxonomy ID: 185431 Superkingdom: Eukaryota Kingdom: not available Genus/species: Trypanosoma Trypanosoma brucei
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Centrin, putative: MAALTDEQIREAFNLFDADG
SGAIDAEEMALAMKGLGFGD
LPRDEVERTVRSMNTNANGL
IEYGEFERMVKSRMAQKDSP
EEVLKAFQLFDLDKKGKISF
ANLKEVAKLLGENPGDDVLQ
EMIAEADEDGDGEVSFEEFK
SVMMQMRGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 368 |
| 15N chemical shifts | 145 |
| 1H chemical shifts | 960 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 149 residues - 16570.627 Da.
| 1 | MET | ALA | ALA | LEU | THR | ASP | GLU | GLN | ILE | ARG | ||||
| 2 | GLU | ALA | PHE | ASN | LEU | PHE | ASP | ALA | ASP | GLY | ||||
| 3 | SER | GLY | ALA | ILE | ASP | ALA | GLU | GLU | MET | ALA | ||||
| 4 | LEU | ALA | MET | LYS | GLY | LEU | GLY | PHE | GLY | ASP | ||||
| 5 | LEU | PRO | ARG | ASP | GLU | VAL | GLU | ARG | THR | VAL | ||||
| 6 | ARG | SER | MET | ASN | THR | ASN | ALA | ASN | GLY | LEU | ||||
| 7 | ILE | GLU | TYR | GLY | GLU | PHE | GLU | ARG | MET | VAL | ||||
| 8 | LYS | SER | ARG | MET | ALA | GLN | LYS | ASP | SER | PRO | ||||
| 9 | GLU | GLU | VAL | LEU | LYS | ALA | PHE | GLN | LEU | PHE | ||||
| 10 | ASP | LEU | ASP | LYS | LYS | GLY | LYS | ILE | SER | PHE | ||||
| 11 | ALA | ASN | LEU | LYS | GLU | VAL | ALA | LYS | LEU | LEU | ||||
| 12 | GLY | GLU | ASN | PRO | GLY | ASP | ASP | VAL | LEU | GLN | ||||
| 13 | GLU | MET | ILE | ALA | GLU | ALA | ASP | GLU | ASP | GLY | ||||
| 14 | ASP | GLY | GLU | VAL | SER | PHE | GLU | GLU | PHE | LYS | ||||
| 15 | SER | VAL | MET | MET | GLN | MET | ARG | GLY | LYS |
Samples:
sample_1: centrin4, [U-13C; U-15N], 0.5 mM; sodium chloride 200 mM; sodium phosphate 20 mM; DTT 2 mM; EDTA 2 mM; H2O 90%; D2O, [U-2H], 10%
sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert P., Guntert, Mumenthaler and Wuthrich - refinement, structure calculation
SPARKY, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker DMX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts