BMRB Entry 4172
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4172
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Title: Response Element of the Orphan Nuclear Receptor Rev-erb Beta
Deposition date: 1998-08-03 Original release date: 2000-12-14
Authors: Castagne, C.; Terenzi, H.; Zakin, M.; Delepierre, M.
Citation: Castagne, C.; Terenzi, H.; Zakin, M.; Delepierre, M.. "Solution Structure of the Orphan Nuclear Receptor Rev-erb Beta Response Element by 1H, 31P NMR and Molecular Simulation" Biochimie 82, 739-748 (2000).
Assembly members:
Rev-RE subunit 1, polymer, 15 residues, Formula weight is not available
Rev-RE subunit 2, polymer, 15 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Rev-RE subunit 1: TAGAATGTAGGTCAG
Rev-RE subunit 2: CTGACCTACATTCTA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 268 |
| 31P chemical shifts | 28 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rev-RE subunit 1 | 1 |
| 2 | Rev-RE subunit 2 | 2 |
Entities:
Entity 1, Rev-RE subunit 1 15 residues - Formula weight is not available
| 1 | DT | DA | DG | DA | DA | DT | DG | DT | DA | DG | ||||
| 2 | DG | DT | DC | DA | DG |
Entity 2, Rev-RE subunit 2 15 residues - Formula weight is not available
| 1 | DC | DT | DG | DA | DC | DC | DT | DA | DC | DA | ||||
| 2 | DT | DT | DC | DT | DA |
Samples:
sample_one: Rev-RE subunit 1 3.6 mM; Rev-RE subunit 2 3.6 mM
sample_conditions_one: pH: 7.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY TOCSY | sample_one | not available | sample_conditions_one |
| 31P-1H hcosyinv and hsqc-tocsy | sample_one | not available | sample_conditions_one |
Software:
FELIX v95.0 - cross-peaks volume calculation
JUMNA v8.0 - molecular simulation nucleic acid minimization
NMR spectrometers:
- VARIAN UNITY 500 MHz