BMRB Entry 4201
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4201
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Title: Role of the 6-20 Disulfide Bridge in the Structure and Activity of Epidermal Growth Factor
Deposition date: 1998-09-14 Original release date: 2000-03-08
Authors: Barnham, K.; Torres, A.; Alewood, D.; Alewood, P.; Domagala, T.; Nice, E.; Norton, R.
Citation: Barnham, K.; Torres, A.; Alewood, D.; Alewood, P.; Domagala, T.; Nice, E.; Norton, R.. "Role of the 6-20 Disulfide Bridge in the Structure and Activity of Epidermal Growth Factor" Protein Sci. 7, 1738-1749 (1998).
Assembly members:
[ABA6,20]_mEGF4-48, polymer, 45 residues, 103.120 Da.
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
[ABA6,20]_mEGF4-48: PGXPSSYDGYCLNGGVXMHI
ESLDSYTCNCVIGYSGDRCQ
TRDLR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 244 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | [ABA6,20] mEGF4-48 | 1 |
Entities:
Entity 1, [ABA6,20] mEGF4-48 45 residues - 103.120 Da.
| 1 | PRO | GLY | ABA | PRO | SER | SER | TYR | ASP | GLY | TYR | ||||
| 2 | CYS | LEU | ASN | GLY | GLY | VAL | ABA | MET | HIS | ILE | ||||
| 3 | GLU | SER | LEU | ASP | SER | TYR | THR | CYS | ASN | CYS | ||||
| 4 | VAL | ILE | GLY | TYR | SER | GLY | ASP | ARG | CYS | GLN | ||||
| 5 | THR | ARG | ASP | LEU | ARG |
Samples:
sample_one: [ABA6,20]_mEGF4-48 mM
sample_conditions_one: pH: 2.8 na; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker AMX 600 MHz
Related Database Links:
| PDB |