BMRB Entry 4207
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4207
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Title: CRO Repressor Insertion Mutant K56-[DGEVK]
Deposition date: 1998-10-11 Original release date: 2002-07-12
Authors: Mossing, M.
Citation: Mossing, M.. "Solution Structure and Dynamics of a Designed Monomeric Variant of the Lambda CRO Repressor" Protein Sci. 7, 983-993 (1998).
Assembly members:
CRO Repressor Insertion Mutant K56-[DGEVK], polymer, 71 residues, Formula weight is not available
Natural source: Common Name: bacteriophage lambda Taxonomy ID: 10710 Superkingdom: not available Kingdom: not available Genus/species: Lambda phage group bacteriophage lambda
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
CRO Repressor Insertion Mutant K56-[DGEVK]: MEQRITLKDYAMRFGQTKTA
KDLGVYQSAINKAIHAGRKI
FLTINADGSVYAEEVKDGEV
KPFPSNKKTTA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 434 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CRO-K56-[DGEVK] | 1 |
Entities:
Entity 1, CRO-K56-[DGEVK] 71 residues - Formula weight is not available
| 1 | MET | GLU | GLN | ARG | ILE | THR | LEU | LYS | ASP | TYR | ||||
| 2 | ALA | MET | ARG | PHE | GLY | GLN | THR | LYS | THR | ALA | ||||
| 3 | LYS | ASP | LEU | GLY | VAL | TYR | GLN | SER | ALA | ILE | ||||
| 4 | ASN | LYS | ALA | ILE | HIS | ALA | GLY | ARG | LYS | ILE | ||||
| 5 | PHE | LEU | THR | ILE | ASN | ALA | ASP | GLY | SER | VAL | ||||
| 6 | TYR | ALA | GLU | GLU | VAL | LYS | ASP | GLY | GLU | VAL | ||||
| 7 | LYS | PRO | PHE | PRO | SER | ASN | LYS | LYS | THR | THR | ||||
| 8 | ALA |
Samples:
sample_one: CRO Repressor Insertion Mutant K56-[DGEVK] mM; H2O 90%; D2O 10%
sample_conditions_one: ionic strength: 0.02 M; pH: 4.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_one | not available | not available |
| COSY | sample_one | not available | not available |
| TOCSY | sample_one | not available | not available |
| HSQC-NOESY | sample_one | not available | not available |
| HSQC-TOCSY | sample_one | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian UNITY-plus 500 MHz
- Varian UNITY-plus 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts