BMRB Entry 4209
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4209
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Title: Solution NMR structures of the major coat protein of filamentous bacteriophage M13 solubilized in Dodecyl Phosphocholine micelles, 25 lowest energy structures
Deposition date: 1998-04-17 Original release date: 2000-02-03
Authors: Papavoine, C.; Christiaans, B.; Folmer, R.; Konings, R.; Hilbers, C.
Citation: Papavoine, C.; Christiaans, B.; Folmer, R.; Konings, R.; Hilbers, C.. "Solution structure of the M13 major coat protein in detergent micelles: A basis for a model of phage assembly involving specific residues" J. Mol. Biol. 282, 401-419 (1998).
Assembly members:
M13 major coat protein, polymer, 50 residues, Formula weight is not available
Natural source: Common Name: bacteriophage M13 Taxonomy ID: 10870 Superkingdom: viruses Kingdom: not available Genus/species: Inoviridae Coliphage M13
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
M13 major coat protein: AEGDDPAKAAFNSLQASATE
YIGYAWAMVVVIVGATIGIK
LFKKFTSKAS
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 157 |
| 15N chemical shifts | 50 |
| 1H chemical shifts | 317 |
| coupling constants | 29 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | gVIIIp | 1 |
Entities:
Entity 1, gVIIIp 50 residues - Formula weight is not available
| 1 | ALA | GLU | GLY | ASP | ASP | PRO | ALA | LYS | ALA | ALA | |
| 2 | PHE | ASN | SER | LEU | GLN | ALA | SER | ALA | THR | GLU | |
| 3 | TYR | ILE | GLY | TYR | ALA | TRP | ALA | MET | VAL | VAL | |
| 4 | VAL | ILE | VAL | GLY | ALA | THR | ILE | GLY | ILE | LYS | |
| 5 | LEU | PHE | LYS | LYS | PHE | THR | SER | LYS | ALA | SER |
Samples:
sample_1: M13 major coat protein, [U-100% 13C; U-100% 15N], 1.5 – 2 mM; dodecyl phosphocholine
sample_cond_1: pH: 4.9; temperature: 311 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| NOESY-HMQC (15N and 13C) | not available | not available | not available |
| TOCSY-HMQC (15N) | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| ROESY-GHSQC | not available | not available | not available |
| HMQC-NOESY-GHSQC | not available | not available | not available |
| HMQC-J | not available | not available | not available |
| HNHA | not available | not available | not available |
| HACACB-COSY | not available | not available | not available |
| HNHB | not available | not available | not available |
| HSQC(3J CGN) | not available | not available | not available |
| HSQC(3JCGC') | not available | not available | not available |
Software:
MNMR - FT of the recorded spectra
XEASY v3.9.1 - Analysis of the spectra to obtain assignments and structural information
NMR spectrometers:
- Bruker AM 400 MHz
- Bruker AM 500 MHz
- Varian Unity+ 500 MHz
- Bruker AMX 600 MHz
Related Database Links:
| BMRB | 17728 19734 19747 2590 2591 2592 4197 |
| PDB | |
| EMBL | CAA23861 CAA23871 |
| GB | AAA32207 AAA32214 AAA32220 AAA32308 AAB24445 |
| PRF | 0812197K |
| REF | NP_510890 YP_009111292 YP_009111302 |
| SP | P69539 P69540 P69541 |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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