BMRB Entry 4223
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4223
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Title: Solution Structure of a TBP-TAFII230 Complex: Protein Mimicry of the Minor Groove Surface of the TATA Box Unwound by TBP
Deposition date: 1998-08-16 Original release date: 1999-12-06
Authors: Liu, D.; Ishima, R.; Tong, K.; Baby, S.; Kokubo, T.; Muhandiram, D.; Kay, L.; Nakatani, Y.; Ikura, M.
Citation: Liu, Dingjiang; Ishima, R.; Tong, K.; Bagby, S.; Kokubo, T.; Muhandiram, D.; Kay, L.; Nakatani, Y.; Ikura, M.. "Solution Structure of a TBP-TAFII230 Complex: Protein Mimicry of the Minor Groove Surface of the TATA Box Unwound by TB" Cell 94, 573-583 (1998).
Assembly members:
TATA box binding protein associated factor, polymer, 67 residues, Formula weight is not available
Natural source: Common Name: Drosophila Taxonomy ID: 7215 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TATA box binding protein associated factor: EGSIGNGLDLTGILFGNIDS
EGRLLQDDDGEGRGGTGFDA
ELRENIGSLSKLGLDSMLLE
VIDLKEA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 239 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 400 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TAFII230 | 1 |
Entities:
Entity 1, TAFII230 67 residues - Formula weight is not available
| 1 | GLU | GLY | SER | ILE | GLY | ASN | GLY | LEU | ASP | LEU | ||||
| 2 | THR | GLY | ILE | LEU | PHE | GLY | ASN | ILE | ASP | SER | ||||
| 3 | GLU | GLY | ARG | LEU | LEU | GLN | ASP | ASP | ASP | GLY | ||||
| 4 | GLU | GLY | ARG | GLY | GLY | THR | GLY | PHE | ASP | ALA | ||||
| 5 | GLU | LEU | ARG | GLU | ASN | ILE | GLY | SER | LEU | SER | ||||
| 6 | LYS | LEU | GLY | LEU | ASP | SER | MET | LEU | LEU | GLU | ||||
| 7 | VAL | ILE | ASP | LEU | LYS | GLU | ALA |
Samples:
sample_1: TATA box binding protein associated factor, [U-13C; U-15N; U60%-2H], 1.0 mM
sample_2: TATA box binding protein associated factor, [U-13C; U-15N], 1.0 mM
sample_cond_1: ionic strength: 0.75 M; pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | sample_cond_1 |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| PDB | |
| GB | AAB26991 AAD19815 AAF54102 AAO47866 AAS65116 |
| REF | NP_001163532 NP_001246952 NP_001287201 NP_476956 NP_996159 |
| SP | P51123 |
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