BMRB Entry 4225
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4225
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Title: NMR structure of Escherichia coli glutaredoxin3-glutathione mixed disulfide complex
Deposition date: 1998-08-17 Original release date: 2000-03-09
Authors: Nordstrand, K.; Aslund, F.; Holmgren, A.; Otting, G.; Berndt, K.
Citation: Nordstrand, K.; Aslund, F.; Holmgren, A.; Otting, G.; Berndt, K.. "NMR Structure of Escherichia coli Glutaredoxin 3-glutathione Mixed Disulfide Complex: Implications for the Enzymatic Mechanism" J. Mol. Biol. 286, 541-552 (1999).
Assembly members:
Glutaredoxin 3, polymer, 83 residues, Formula weight is not available
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Glutaredoxin 3: ANVEIYTKETCPYSHRAKAL
LSSKGVSFQELPIDGNAAKR
EEMIKRSGRTTVPQIFIDAQ
HIGGYDDLYALDARGGLDPL
LKX
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 87 |
| 1H chemical shifts | 501 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Glutaredoxin 3 | 1 |
Entities:
Entity 1, Glutaredoxin 3 83 residues - Formula weight is not available
| 1 | ALA | ASN | VAL | GLU | ILE | TYR | THR | LYS | GLU | THR | ||||
| 2 | CYS | PRO | TYR | SER | HIS | ARG | ALA | LYS | ALA | LEU | ||||
| 3 | LEU | SER | SER | LYS | GLY | VAL | SER | PHE | GLN | GLU | ||||
| 4 | LEU | PRO | ILE | ASP | GLY | ASN | ALA | ALA | LYS | ARG | ||||
| 5 | GLU | GLU | MET | ILE | LYS | ARG | SER | GLY | ARG | THR | ||||
| 6 | THR | VAL | PRO | GLN | ILE | PHE | ILE | ASP | ALA | GLN | ||||
| 7 | HIS | ILE | GLY | GLY | TYR | ASP | ASP | LEU | TYR | ALA | ||||
| 8 | LEU | ASP | ALA | ARG | GLY | GLY | LEU | ASP | PRO | LEU | ||||
| 9 | LEU | LYS | GSH |
Samples:
sample_1: Glutaredoxin 3 2.8 mM
sample_2: Glutaredoxin 3, [U-15N], 5.0 mM
sample_cond_1: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 301 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | sample_cond_1 |
Software:
PROSA v3.6 - spectral FT-transformations; baseline correction
XEASY v1.2 - spectral analysis
NMR spectrometers:
- Bruker . 600 MHz
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