BMRB Entry 4227
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4227
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Title: NMR structures (20) of the J-domain (residues 1-77) of the Escherichia coli N-terminal fragment (residues 1-78) of the Molecular Chaperone DNAJ
Deposition date: 1998-08-20 Original release date: 2001-07-09
Authors: Huang, K.; Flanagan, J.; Prestegard, J.
Citation: Huang, K.; Flanagan, J.; Prestegard, J.. "The influence of C-terminal extension on the structure of the J-domain in E. Coli DNAJ" Protein Sci. 8, 203-214 (1999).
Assembly members:
DNAJ, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: Proteobacteria Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
DNAJ: AKQDYYEILGVSKTAEEREI
RKAYKRLAMKYHPDRNQGDK
EAEAKFKEIKEAYEVLTDSQ
KRAAYDQYGHAAFEQGGM
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 341 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 525 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNAJ | 1 |
Entities:
Entity 1, DNAJ 78 residues - Formula weight is not available
| 1 | ALA | LYS | GLN | ASP | TYR | TYR | GLU | ILE | LEU | GLY | ||||
| 2 | VAL | SER | LYS | THR | ALA | GLU | GLU | ARG | GLU | ILE | ||||
| 3 | ARG | LYS | ALA | TYR | LYS | ARG | LEU | ALA | MET | LYS | ||||
| 4 | TYR | HIS | PRO | ASP | ARG | ASN | GLN | GLY | ASP | LYS | ||||
| 5 | GLU | ALA | GLU | ALA | LYS | PHE | LYS | GLU | ILE | LYS | ||||
| 6 | GLU | ALA | TYR | GLU | VAL | LEU | THR | ASP | SER | GLN | ||||
| 7 | LYS | ARG | ALA | ALA | TYR | ASP | GLN | TYR | GLY | HIS | ||||
| 8 | ALA | ALA | PHE | GLU | GLN | GLY | GLY | MET |
Samples:
sample_1: DNAJ mM; phosphate 50 mM
sample_cond_1: pH: 6.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Varian Unity 600 MHz
- GE Omega 500 MHz
Related Database Links:
| BMRB | 4228 |
| PDB | |
| DBJ | BAB33438 BAB96590 BAG75538 BAI23378 BAI28898 |
| EMBL | CAP74585 CAQ30532 CAQ87599 CAQ96906 CAR01382 |
| GB | AAA00009 AAA23693 AAC73126 AAG54315 AAN41681 |
| REF | NP_308042 NP_414556 NP_705974 WP_000240600 WP_001118445 |
| SP | A7ZHA5 A7ZVV8 B1IRF9 B1LFU5 B1XBE0 |
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