BMRB Entry 4241
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4241
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H and 15N Assignments of Internal xylan binding domain from XYLD
Deposition date: 1998-10-09 Original release date: 1999-11-29
Authors: Simpson, Peter; Bolam, David; Cooper, Alan; Ciruela, Antonio; Hazlewood, Geoffrey; Gilbert, Harry; Williamson, Michael
Citation: Simpson, Peter; Bolam, David; Cooper, Alan; Ciruela, Antonio; Hazlewood, Geoffrey; Gilbert, Harry; Williamson, Michael. "The Three-Dimensional Structure of a Family IIb Xylan Binding Domain: Evidence that the Orientation of the Solvent-Exposed Tryptophans Determines the Ligand Specificity of Type II Polysaccharide Binding Domains." Structure 7, 853-864 (1999).
Assembly members:
xylanase D, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 1708 Superkingdom: eubacteria Kingdom: not available Genus/species: Cellulomonas fimi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
xylanase D: TGCSVTATRAEEWSDRFNVT
YSVSGSSAWTVNLALNGSQT
IQASWNANVTGSGSTRTVTP
NGSGNTFGVTVMKNGSSTTP
AATCAGS
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 100 |
| 1H chemical shifts | 532 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | XBD1-XYLD | 1 |
Entities:
Entity 1, XBD1-XYLD 87 residues - Formula weight is not available
| 1 | THR | GLY | CYS | SER | VAL | THR | ALA | THR | ARG | ALA | ||||
| 2 | GLU | GLU | TRP | SER | ASP | ARG | PHE | ASN | VAL | THR | ||||
| 3 | TYR | SER | VAL | SER | GLY | SER | SER | ALA | TRP | THR | ||||
| 4 | VAL | ASN | LEU | ALA | LEU | ASN | GLY | SER | GLN | THR | ||||
| 5 | ILE | GLN | ALA | SER | TRP | ASN | ALA | ASN | VAL | THR | ||||
| 6 | GLY | SER | GLY | SER | THR | ARG | THR | VAL | THR | PRO | ||||
| 7 | ASN | GLY | SER | GLY | ASN | THR | PHE | GLY | VAL | THR | ||||
| 8 | VAL | MET | LYS | ASN | GLY | SER | SER | THR | THR | PRO | ||||
| 9 | ALA | ALA | THR | CYS | ALA | GLY | SER |
Samples:
sample_one: xylanase D, [U-99% 15N], 1.0 mM; sodium_phosphate 50 mM
sample_conditions_one: pH: 5.0; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| COSY | sample_one | not available | not available |
| NOESY | sample_one | not available | not available |
| TOCSY | sample_one | not available | not available |
| E. COSY | sample_one | not available | not available |
| 1H-15N HSQC | sample_one | not available | not available |
| NOESY-HMQC | sample_one | not available | not available |
| TOCSY-HMQC | sample_one | not available | not available |
| HNHA | sample_one | not available | not available |
| HNHB | sample_one | not available | not available |
Software:
FELIX v97.0 -
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts