BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 4248

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4248

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Title: LEF1 HMG Domain (From Mouse), Complexed with DNA (15bp), NMR, 12 Structures

Deposition date: 1998-10-16 Original release date: 1999-11-23

Authors: Li, X.; Love, J.; Case, D.; Wright, P.

Citation: Love, J.; Li, X.; Case, D.; Giese, K.; Grosschedl, R.; Wright, P.. "Structural basis for DNA bending by the architectural transcription factor LEF-1"  Nature 376, 791-. (1995).

Assembly members:
Lymphoid enhancer-binding factor, polymer, 86 residues, Formula weight is not available
DNA, polymer, 15 residues, Formula weight is not available
DNA, polymer, 15 residues, Formula weight is not available

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Lymphoid enhancer-binding factor: MHIKKPLNAFMLYMKEMRAN VVAESTLKESAAINQILGRR WHALSREEQAKYYELARKER QLHMQLYPGWSARDNYGKKK KRKREK
DNA: CACCCTTTGAAGCTC
DNA: GAGCTTCAAAGGGTG

Data typeCount
13C chemical shifts309
15N chemical shifts88
1H chemical shifts815

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LEF-1(HMG)1
2DNA_chain_B2
3DNA_chain_C3

Entities:

Entity 1, LEF-1(HMG) 86 residues - Formula weight is not available

1   METHISILELYSLYSPROLEUASNALAPHE
2   METLEUTYRMETLYSGLUMETARGALAASN
3   VALVALALAGLUSERTHRLEULYSGLUSER
4   ALAALAILEASNGLNILELEUGLYARGARG
5   TRPHISALALEUSERARGGLUGLUGLNALA
6   LYSTYRTYRGLULEUALAARGLYSGLUARG
7   GLNLEUHISMETGLNLEUTYRPROGLYTRP
8   SERALAARGASPASNTYRGLYLYSLYSLYS
9   LYSARGLYSARGGLULYS

Entity 2, DNA_chain_B 15 residues - Formula weight is not available

1   DCDADCDCDCDTDTDTDGDA
2   DADGDCDTDC

Entity 3, DNA_chain_C 15 residues - Formula weight is not available

1   DGDADGDCDTDTDCDADADA
2   DGDGDGDTDG

Samples:

sample_one: Lymphoid enhancer-binding factor, [U-95% 13C; U-95% 15N], 1.2 mM

sample_conditions_one: ionic strength: 0.01 M; pH: 6.7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
CT-HNCAsample_onenot availablesample_conditions_one
CBCA(CO)NHsample_onenot availablesample_conditions_one
HNCACBsample_onenot availablesample_conditions_one
C(CO)NH-TOCSYsample_onenot availablesample_conditions_one
HCCH-COSYsample_onenot availablesample_conditions_one
HCCH-TOCSYsample_onenot availablesample_conditions_one
(15N)3D NOESY-HSQCsample_onenot availablesample_conditions_one
(13C)3D NOESY-HSQCsample_onenot availablesample_conditions_one
(13C/13C)4D HMQC-NOESY-HMQCsample_onenot availablesample_conditions_one
2D 13C/15N DOUBLE HALF-FILTERED NOESYsample_onenot availablesample_conditions_one
3D 13C-SELECTED(W1,W2) 12C-FILTERED (W3) NOESYsample_onenot availablesample_conditions_one
2D 1H NOESY, AROMATIC 13C CT-HSQCsample_onenot availablesample_conditions_one
HNCA-Jsample_onenot availablesample_conditions_one
HNHAsample_onenot availablesample_conditions_one
HNHBsample_onenot availablesample_conditions_one
HACAHB-COSYsample_onenot availablesample_conditions_one
HMBCsample_onenot availablesample_conditions_one
3D LRCCsample_onenot availablesample_conditions_one

Software:

FELIX v2.30 -

DIANA v2.80 -

AMBER v4.10 -

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker AMX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
DBJ BAA03954 BAC25837 BAC28841 BAC38867 BAD74116
EMBL CAA41493 CAH56421
GB AAC24524 AAD16968 AAD41489 AAD41490 AAF13268
PIR B39625
REF NP_001082124 NP_001083866 NP_001090203 NP_001123439 NP_001124185
SP P27782 Q9QXN1 Q9UJU2
TPG DAA28899

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts