BMRB Entry 4281
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4281
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Title: Backbone and Side Chain 1H, 13C, and 15N Chemical Shift Assignments for AbrBN
Deposition date: 1998-12-10 Original release date: 2005-08-05
Authors: Bobay, Benjamin; Andreeva, Antonina; Mueller, Geoffrey; Cavanagh, John; Murzin, Alexey
Citation: Bobay, Benjamin; Andreeva, Antonina; Mueller, Geoffrey; Cavanagh, John; Murzin, Alexey. "Revised structure of the AbrB N-terminal domain unifies a diverse superfamily of putative DNA-binding proteins" . ., .-..
Assembly members:
AbrB, polymer, 53 residues, 6100 Da.
Natural source: Common Name: B. subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
AbrB: MKSTGIVRKVDELGRVVIPI
ELRRTLGIAEKDALEIYVDD
EKIILKKYKPNMT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 403 |
| 13C chemical shifts | 238 |
| 15N chemical shifts | 54 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AbrBN subunit 1 | 1 |
| 2 | AbrBN subunit 2 | 1 |
Entities:
Entity 1, AbrBN subunit 1 53 residues - 6100 Da.
| 1 | MET | LYS | SER | THR | GLY | ILE | VAL | ARG | LYS | VAL | ||||
| 2 | ASP | GLU | LEU | GLY | ARG | VAL | VAL | ILE | PRO | ILE | ||||
| 3 | GLU | LEU | ARG | ARG | THR | LEU | GLY | ILE | ALA | GLU | ||||
| 4 | LYS | ASP | ALA | LEU | GLU | ILE | TYR | VAL | ASP | ASP | ||||
| 5 | GLU | LYS | ILE | ILE | LEU | LYS | LYS | TYR | LYS | PRO | ||||
| 6 | ASN | MET | THR |
Samples:
sample_one: AbrB, [U-99% 13C; U-99% 15N], 1.5 – 2.5 mM; KCl 15 mM; K-PO 10 mM; DTT 1 mM; NaN3 0.02%; D20 5%; H2O 95%
sample_two: AbrB, [U-99% 13C; U-99% 15N], 1.5 – 2.5 mM; KCl 15 mM; K-PO 10 mM; DTT 1 mM; NaN3 0.02%; D20 5%; H2O 95%
standard_h2o_conditions: pH: 5.8; temperature: 305 K
standard_d2o_conditions: pH*: 5.8; temperature: 305 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY (120mS and 150mS mixing time) | not available | not available | not available |
| 1H-13C COESY (120mS and 120mS folded mixing time) | not available | not available | not available |
| 3-D HNCA | not available | not available | not available |
| 3-D HN(CO)CA | not available | not available | not available |
| 3-D HNCO | not available | not available | not available |
| 3-D HN(CA)CO | not available | not available | not available |
| 3-D CBCA(CO)NH | not available | not available | not available |
| 3-D HNCACB | not available | not available | not available |
| D2O exchange via 2-D 1H-15N HSQC | not available | not available | not available |
| CC-TOCSY-(CO)NH | not available | not available | not available |
| H(CC-TOCSY-CO)NH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
Software:
ARIA v1.2 -
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 17650 |
| PDB | |
| DBJ | BAA05272 BAI83483 BAL15827 BAM48968 BAM56238 |
| EMBL | CAA31307 CAA43955 CAB11813 CBI41163 CCF03572 |
| GB | AAA22195 AAP07140 AAP24090 AAS38971 AAT29114 |
| PRF | 1715209A |
| REF | NP_387918 NP_829939 NP_842604 WP_000843034 WP_000843035 |
| SP | P08874 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts