BMRB Entry 4351
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4351
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Title: A 30-residue Fragment of the Carp Granulin-1 Protein Folds into a Stack of two Beta-hairpins Similar to that found in the Native Protein PubMed: 10424355
Deposition date: 1999-06-03 Original release date: 2001-03-15
Authors: Vranken, Wim; Chen, Z.; Xu, Ping; James, Susan; Bennett, Hugh; Ni, Feng
Citation: Vranken, Wim; Chen, Z.; Xu, Ping; James, Susan; Bennett, Hugh; Ni, Feng. "A 30-residue Fragment of the Carp Granulin-1 Protein Folds into a Stack of two Beta-hairpins Similar to that found in the Native Protein" J. Pept. Res. 53, 590-597 (1999).
Assembly members:
granulin, polymer, 30 residues, Formula weight is not available
Natural source: Common Name: carp Taxonomy ID: 7962 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Cyprinus carpio
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
granulin: VIHCDAATICPDGTTCSLSP
YGVWYCSPFS
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 53 |
| 1H chemical shifts | 181 |
| coupling constants | 5 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GRN1 CYPCA | 1 |
Entities:
Entity 1, GRN1 CYPCA 30 residues - Formula weight is not available
| 1 | VAL | ILE | HIS | CYS | ASP | ALA | ALA | THR | ILE | CYS | |
| 2 | PRO | ASP | GLY | THR | THR | CYS | SER | LEU | SER | PRO | |
| 3 | TYR | GLY | VAL | TRP | TYR | CYS | SER | PRO | PHE | SER |
Samples:
water_sample: granulin 0.54 mM
standard: ionic strength: 0.02 M; pH: 4.5; pressure: 1 atm; temperature: 288.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY | water_sample | not available | standard |
| NOESY | water_sample | not available | standard |
| TOCSY spinlock with MLEV-17 | water_sample | not available | standard |
| NOESY using water flipback | water_sample | not available | standard |
| Water suppression with WATERGATE | water_sample | not available | standard |
Software:
XWIN-NMR - Spectrum processing
PRONTO - Assignment of spectra and keeping record of NOE contacts
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz
Related Database Links:
| PDB |