BMRB Entry 4394
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4394
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Title: Translation initiation factor IF3 from Escherichia coli Ribosome binding domain (residues 84-180)
Deposition date: 1999-09-09 Original release date: 1999-11-29
Authors: DE COCK, E.; GARCIA, C.; DARDEL, F.
Citation: DE COCK, E.; BLANQUET, S.; LALLEMAND, J.; DARDEL, F.. "INTERACTION OF E. COLI TRANSLATION INITIATION FACTOR IF3 WITH THE RIBOSOME" . ., .-..
Assembly members:
TRANSLATION INITIATION FACTOR IF3, polymer, 100 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: ESCHERICHIA COLI
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI
Entity Sequences (FASTA):
TRANSLATION INITIATION FACTOR IF3: MEFQKKKQKVIQVKEIKFRP
GTDEGDYQVKLRSLIRFLEE
GDKAKITLRFRGREMAHQQI
GMEVLNRVKDDLQELAVVES
FPTKIEGRQMIMVLAPKKKQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 256 |
| 15N chemical shifts | 92 |
| 1H chemical shifts | 637 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | IF3 ribosome binding domain | 1 |
Entities:
Entity 1, IF3 ribosome binding domain 100 residues - Formula weight is not available
| 1 | MET | GLU | PHE | GLN | LYS | LYS | LYS | GLN | LYS | VAL | |
| 2 | ILE | GLN | VAL | LYS | GLU | ILE | LYS | PHE | ARG | PRO | |
| 3 | GLY | THR | ASP | GLU | GLY | ASP | TYR | GLN | VAL | LYS | |
| 4 | LEU | ARG | SER | LEU | ILE | ARG | PHE | LEU | GLU | GLU | |
| 5 | GLY | ASP | LYS | ALA | LYS | ILE | THR | LEU | ARG | PHE | |
| 6 | ARG | GLY | ARG | GLU | MET | ALA | HIS | GLN | GLN | ILE | |
| 7 | GLY | MET | GLU | VAL | LEU | ASN | ARG | VAL | LYS | ASP | |
| 8 | ASP | LEU | GLN | GLU | LEU | ALA | VAL | VAL | GLU | SER | |
| 9 | PHE | PRO | THR | LYS | ILE | GLU | GLY | ARG | GLN | MET | |
| 10 | ILE | MET | VAL | LEU | ALA | PRO | LYS | LYS | LYS | GLN |
Samples:
sample_1: TRANSLATION INITIATION FACTOR IF3, [U-98% 13C; U-95% 15N], 1.5 mM; potassium phosphate 20 mM
sample_2: TRANSLATION INITIATION FACTOR IF3, [U-98% 15N], 1.5 mM; potassium phosphate 20 mM
sample_cond_1: ionic strength: 20 mM; pH*: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-13C-1H HCCH-TOCSY | not available | not available | sample_cond_1 |
| 3D HNCA | not available | not available | sample_cond_1 |
| 3D HNHB | not available | not available | sample_cond_1 |
| 3D 1H-1H-15N NOESY | not available | not available | sample_cond_1 |
| 3D 13C-1H-1H NOESY | not available | not available | sample_cond_1 |
Software:
GIFA v4.0 - NMR data processing and graphic display
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA15485 BAB35848 BAG66478 BAG77410 BAH63902 |
| EMBL | CAA23561 CAD02019 CAP76214 CAQ32193 CAR33292 |
| GB | AAA51467 AAC36811 AAC36814 AAC74788 AAG56705 |
| PIR | AB0706 E85780 |
| REF | NP_310452 NP_416233 NP_456178 NP_460300 NP_707396 |
| SP | B1IPL0 B7USA1 C4ZYI0 P0A707 P0A708 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts