BMRB Entry 4428
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4428
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Title: Solution Structure of the Headpiece Domain of Chicken Villin PubMed: 10600386
Deposition date: 1999-10-02 Original release date: 2001-07-30
Authors: Vardar, D.; Buckley, D.; Frank, B.; McKnight, C.
Citation: Vardar, D.; Buckley, D.; Frank, B.; McKnight, C.. "NMR structure of an F-actin-binding "headpiece" motif from villin" J. Mol. Biol. 294, 1299-1310 (1999).
Assembly members:
HEADPIECE DOMAIN FROM CHICKEN VILLIN, polymer, 67 residues, Formula weight is not available
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: .
Entity Sequences (FASTA):
HEADPIECE DOMAIN FROM CHICKEN VILLIN: PTKLETFPLDVLVNTAAEDL
PRGVDPSRKENHLSDEDFKA
VFGMTRSAFANLPLWKQQNL
KKEKGLF
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 456 |
| 15N chemical shifts | 69 |
| coupling constants | 42 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HEADPIECE DOMAIN FROM CHICKEN VILLIN | 1 |
Entities:
Entity 1, HEADPIECE DOMAIN FROM CHICKEN VILLIN 67 residues - Formula weight is not available
| 1 | PRO | THR | LYS | LEU | GLU | THR | PHE | PRO | LEU | ASP | ||||
| 2 | VAL | LEU | VAL | ASN | THR | ALA | ALA | GLU | ASP | LEU | ||||
| 3 | PRO | ARG | GLY | VAL | ASP | PRO | SER | ARG | LYS | GLU | ||||
| 4 | ASN | HIS | LEU | SER | ASP | GLU | ASP | PHE | LYS | ALA | ||||
| 5 | VAL | PHE | GLY | MET | THR | ARG | SER | ALA | PHE | ALA | ||||
| 6 | ASN | LEU | PRO | LEU | TRP | LYS | GLN | GLN | ASN | LEU | ||||
| 7 | LYS | LYS | GLU | LYS | GLY | LEU | PHE |
Samples:
sample_1: HEADPIECE DOMAIN FROM CHICKEN VILLIN 1 mM; phosphate buffer 10 mM
sample_2: HEADPIECE DOMAIN FROM CHICKEN VILLIN, [U-15N], 1 mM; phosphate buffer 10 mM
sample_3: HEADPIECE DOMAIN FROM CHICKEN VILLIN, [U-15N; U-10% 13C], 1 mM; phosphate buffer 10 mM
sample_4: HEADPIECE DOMAIN FROM CHICKEN VILLIN 1 mM
sample_cond_1: pH: 7.0; temperature: 293 K; ionic strength: 10 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | sample_cond_1 |
| 3D 15N-SEPARATED NOESY | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
| E-COSY | not available | not available | sample_cond_1 |
| HNHB | not available | not available | sample_cond_1 |
Software:
NMRPIPE -
X-PLOR -
XWINNMR -
NMRVIEW -
FELIX -
MOLMOL -
NMR spectrometers:
- BRUKER DMX 500 MHz
Related Database Links:
| BMRB | 11519 11520 11521 15097 15245 17698 |
| PDB | |
| GB | AAA49133 |
| REF | NP_990773 XP_003207697 XP_010711916 XP_010711917 |
| SP | P02640 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts