BMRB Entry 4510
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4510
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Title: The Second Type II Module From Human Matrix Metalloproteinase 2
Deposition date: 1999-10-08 Original release date: 2002-07-12
Authors: Briknarova, Klara; Grishaev, Alexander; Banyai, Laszlo; Tordai, Hedvig; Patthy, Laszlo; Llinas, Miguel
Citation: Briknarova, Klara; Grishaev, Alexander; Banyai, Laszlo; Tordai, Hedvig; Patthy, Laszlo; Llinas, Miguel. "The second type II module from human matrix metalloproteinase 2: structure, function and dynamics" Structure 7, 1235-1245 (1999).
Assembly members:
The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2, polymer, 60 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2: TALFTMGGNAEGQPCKFPFR
FQGTSYDSCTTEGRTDGYRW
CGTTEDYDRDKKYGFCPETA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 72 |
| 1H chemical shifts | 401 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 2Fn2/MMP-2 | 1 |
Entities:
Entity 1, 2Fn2/MMP-2 60 residues - Formula weight is not available
| 1 | THR | ALA | LEU | PHE | THR | MET | GLY | GLY | ASN | ALA | |
| 2 | GLU | GLY | GLN | PRO | CYS | LYS | PHE | PRO | PHE | ARG | |
| 3 | PHE | GLN | GLY | THR | SER | TYR | ASP | SER | CYS | THR | |
| 4 | THR | GLU | GLY | ARG | THR | ASP | GLY | TYR | ARG | TRP | |
| 5 | CYS | GLY | THR | THR | GLU | ASP | TYR | ASP | ARG | ASP | |
| 6 | LYS | LYS | TYR | GLY | PHE | CYS | PRO | GLU | THR | ALA |
Samples:
sample_1: The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2 1.3 mM; H2O 90%; D2O 10%
sample_2: The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2, [U-15N], 1 mM; H2O 90%; D2O 10%
sample_3: The Second Fibronectin Type II Module From Human Matrix Metalloproteinase 2, [U-15N], 4 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 0 M; pH: 5.2; pressure: 1 atm; temperature: 298 K
sample_cond_2: ionic strength: 0 M; pH*: 5.1; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D COSY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| E-COSY | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
| 3D HNHB | not available | not available | not available |
| 3D 15N-edited TOCSY | not available | not available | not available |
| 3D 15N-edited NOESY | not available | not available | not available |
| 1N-15N NOE | not available | not available | not available |
| T1 and T2 relaxation | not available | not available | not available |
Software:
xwinnmr v2.0 - experiment acquisition
FELIX v95 - data processing and assignment
X-PLOR v3.851 - structure calculation
QUANTA v96 - REFINEMENT
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts