BMRB Entry 4550
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4550
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Title: NMR structure of the palindromic DNA decamer d(GCGTTAACGC)2
Deposition date: 1999-08-20 Original release date: 2006-02-17
Authors: Smith, J.; Tsui, V.; Chazin, W.; Case, D.
Citation: Smith, J.; Tsui, V.; Chazin, W.; Case, D.. "NMR Structure of the Palindromic DNA Decamer d(GCGTTAACGC)2" The BMRB entry is the only known published source for the data..
Assembly members:
5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3', polymer, 10 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3': GCGTTAACGC
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 83 |
| coupling constants | 18 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | strand 1 | 1 |
| 2 | strand 2 | 1 |
Entities:
Entity 1, strand 1 10 residues - Formula weight is not available
| 1 | DG | DC | DG | DT | DT | DA | DA | DC | DG | DC |
Samples:
sample_1: 5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3' 2 mM; Na3PO4 20 mM; NaCl 50 mM; NaN3 0.1%; D2O 99.9%
sample_2: 5'-d(*GP*CP*GP*TP*TP*AP*AP*CP*GP*C)-3' 2 mM; Na3PO4 20 mM; NaCl 50 mM; NaN3 0.1%; D2O 95%
sample_cond_1: ionic strength: 70 mM; pH: 7; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | sample_cond_1 |
| E-COSY | not available | not available | sample_cond_1 |
| 2D NOESY | not available | not available | sample_cond_1 |
Software:
FELIX v95 - processing
NAB v2.1 - structure calculation
AMBER v5.0 - refinement, iterative matrix relaxation
NMR spectrometers:
- Bruker AMX 500 MHz