BMRB Entry 4557
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4557
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Title: Structure of the active domain of the herpes simplex virus protein ICP47 in water/sodium dodecyl sulfate solution determined by nuclear magnetic resonance spectroscop
Deposition date: 1999-12-11 Original release date: 2001-08-06
Authors: Pfaender, R.; Neumann, L.; Zweckstetter, M.; Seger, C.; Holak, T.; Tampe, R.
Citation: Pfaender, R.; Neumann, L.; Zweckstetter, M.; Seger, C.; Holak, T.; Tampe, R.. "The structure of the active domain of the herpes simplex virus protein ICP47 in water/sodium dodecyl sulfate solution determined by nuclear magnetic resonance spectroscop" Biochemistry 38, 13692-13698 (1999).
Assembly members:
herpes simplex virus protein ICP47, polymer, 33 residues, Formula weight is not available
Natural source: Common Name: herpes simplex virus Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemically synthesized
Entity Sequences (FASTA):
herpes simplex virus protein ICP47: SWALEMADTFLDNMRVGPRT
YADVRDEINKRGR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 179 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ICP47 domain 2-34 | 1 |
Entities:
Entity 1, ICP47 domain 2-34 33 residues - Formula weight is not available
| 1 | SER | TRP | ALA | LEU | GLU | MET | ALA | ASP | THR | PHE | ||||
| 2 | LEU | ASP | ASN | MET | ARG | VAL | GLY | PRO | ARG | THR | ||||
| 3 | TYR | ALA | ASP | VAL | ARG | ASP | GLU | ILE | ASN | LYS | ||||
| 4 | ARG | GLY | ARG |
Samples:
sample_1: herpes simplex virus protein ICP47 4.7 mM; SDS, [U-2H], 560 mM; H2O 90%; D2O 10%
sample_2: herpes simplex virus protein ICP47 4.7 mM; SDS, [U-2H], 560 mM; D2O 100%
sample_cond_1: pH: 7.4; temperature: 307 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| COSY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DRX 600 MHz
- Bruker DMX 500 MHz
- Bruker DMX 600 MHz
- Bruker DMX 750 MHz