BMRB Entry 4583
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4583
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Title: The C-terminal Domain of the RNA Polymerase Alpha Subunit from Thermus Thermophilus PubMed: 10821859
Deposition date: 2000-05-05 Original release date: 2000-12-19
Authors: Wada, T.; Yamazaki, T.; Kyogoku, Y.
Citation: Wada, T.; Yamazaki, T.; Kyogoku, Y.. "The structure and the characteristic DNA binding property of the C-terminal domain of the RNA polymerase alpha subunit from Thermus thermophilus" J. Biol. Chem. 275, 16057-16063 (2000).
Assembly members:
RNA POLYMERASE ALPHA SUBUNIT, polymer, 82 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 274 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermus thermophilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
RNA POLYMERASE ALPHA SUBUNIT: AAPEEAKEPEAPPEQEEELD
LPLEELGLSTRVLHSLKEEG
IESVRALLALNLKDLKNIPG
IGERSLEEIKEALEKKGFTL
KE
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 480 |
| 13C chemical shifts | 251 |
| 15N chemical shifts | 83 |
| coupling constants | 69 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNA POLYMERASE ALPHA SUBUNIT | 1 |
Entities:
Entity 1, RNA POLYMERASE ALPHA SUBUNIT 82 residues - Formula weight is not available
| 1 | ALA | ALA | PRO | GLU | GLU | ALA | LYS | GLU | PRO | GLU | ||||
| 2 | ALA | PRO | PRO | GLU | GLN | GLU | GLU | GLU | LEU | ASP | ||||
| 3 | LEU | PRO | LEU | GLU | GLU | LEU | GLY | LEU | SER | THR | ||||
| 4 | ARG | VAL | LEU | HIS | SER | LEU | LYS | GLU | GLU | GLY | ||||
| 5 | ILE | GLU | SER | VAL | ARG | ALA | LEU | LEU | ALA | LEU | ||||
| 6 | ASN | LEU | LYS | ASP | LEU | LYS | ASN | ILE | PRO | GLY | ||||
| 7 | ILE | GLY | GLU | ARG | SER | LEU | GLU | GLU | ILE | LYS | ||||
| 8 | GLU | ALA | LEU | GLU | LYS | LYS | GLY | PHE | THR | LEU | ||||
| 9 | LYS | GLU |
Samples:
sample_1: RNA POLYMERASE ALPHA SUBUNIT, [U-13C; U-15N], 2 mM; phosphate 20 mM; KCl 30 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 6.0; temperature: 310 K; ionic strength: 50 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
NMRPIPE - data collection
X-PLOR v3.1 - data processing
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA75549 BAA88567 BAD71487 |
| GB | AAS81642 AEG34077 AFH38290 EIA38525 |
| PIR | JE0397 |
| REF | WP_008633361 WP_011173698 WP_014629067 YP_144930 |
| SP | Q5SHR6 Q72I32 Q9Z9H6 |
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