BMRB Entry 4584
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4584
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Title: Solution Structure of the DNA-binding Domain of TraM PubMed: 11258958
Deposition date: 2000-05-05 Original release date: 2001-05-02
Authors: Stockner, T.; Plugariu, C.; Koraimann, G.; Hoegenauer, G.; Bermel, W.; Prytulla, S.; Sterk, H.
Citation: Stockner, T.; Plugariu, C.; Koraimann, G.; Hoegenauer, G.; Bermel, W.; Prytulla, S.; Sterk, H.. "Solution Structure of the DNA-binding Domain of TraM" Biochemistry 40, 3370-3377 (2001).
Assembly members:
R1 PLASMID TRAM PROTEIN, polymer, 55 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
R1 PLASMID TRAM PROTEIN: AKVQAYVSDEIVYKINKIVE
RRRAEGAKSTDVSFSSISTM
LLELGLRVYEAQMER
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 399 |
| 15N chemical shifts | 62 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | R1 PLASMID TRAM PROTEIN | 1 |
Entities:
Entity 1, R1 PLASMID TRAM PROTEIN 55 residues - Formula weight is not available
| 1 | ALA | LYS | VAL | GLN | ALA | TYR | VAL | SER | ASP | GLU | ||||
| 2 | ILE | VAL | TYR | LYS | ILE | ASN | LYS | ILE | VAL | GLU | ||||
| 3 | ARG | ARG | ARG | ALA | GLU | GLY | ALA | LYS | SER | THR | ||||
| 4 | ASP | VAL | SER | PHE | SER | SER | ILE | SER | THR | MET | ||||
| 5 | LEU | LEU | GLU | LEU | GLY | LEU | ARG | VAL | TYR | GLU | ||||
| 6 | ALA | GLN | MET | GLU | ARG |
Samples:
sample_1: R1 PLASMID TRAM PROTEIN 4 mg/ml; phosphate buffer 50 mM; H2O 95%; D2O 5%
sample_2: R1 PLASMID TRAM PROTEIN, [U-15N], 1.3 mg/ml; phosphate buffer 50 mM; H2O 95%; D2O 5%
sample_cond_1: pH: 4.0; temperature: 300 K; ionic strength: 50 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 3D-15N-NOESY-HSQC | not available | not available | not available |
| HNHA | not available | not available | not available |
Software:
XWINNMR v2.5 - collection
NMRPipe - processing
ANSIG v3.3 - data analysis
X-PLOR v3.851 - refinement
MOLMOL v2.6 - data analysis
VNMR v5.2 - collection
NMR spectrometers:
- Varian UnityInova 600 MHz
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Related Database Links:
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts