BMRB Entry 4637
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4637
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Title: SOLUTION STRUCTURE OF THE MATA1 HOMEODOMAIN PubMed: 10955992
Deposition date: 2000-06-07 Original release date: 2001-07-09
Authors: Anderson, J.; Forman, M.; Modleski, S.; Dahlquist, F.; Baxter, S.
Citation: Anderson, J.; Forman, M.; Modleski, S.; Dahlquist, F.; Baxter, S.. "Cooperative ordering in homeodomain-DNA recognition: solution structure and dynamics of the MATa1 homeodomain" Biochemistry 39, 10045-10054 (2000).
Assembly members:
MATING-TYPE PROTEIN A-1, polymer, 61 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota fungi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
MATING-TYPE PROTEIN A-1: KKEKSPKGKSSISPQARAFL
EQVFRRKQSLNSKEKEEVAK
KCGITPLQVRVWFINKRMRS
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 353 |
| 13C chemical shifts | 119 |
| 15N chemical shifts | 58 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MATING-TYPE PROTEIN A-1 | 1 |
Entities:
Entity 1, MATING-TYPE PROTEIN A-1 61 residues - Formula weight is not available
| 1 | LYS | LYS | GLU | LYS | SER | PRO | LYS | GLY | LYS | SER | ||||
| 2 | SER | ILE | SER | PRO | GLN | ALA | ARG | ALA | PHE | LEU | ||||
| 3 | GLU | GLN | VAL | PHE | ARG | ARG | LYS | GLN | SER | LEU | ||||
| 4 | ASN | SER | LYS | GLU | LYS | GLU | GLU | VAL | ALA | LYS | ||||
| 5 | LYS | CYS | GLY | ILE | THR | PRO | LEU | GLN | VAL | ARG | ||||
| 6 | VAL | TRP | PHE | ILE | ASN | LYS | ARG | MET | ARG | SER | ||||
| 7 | LYS |
Samples:
sample_1: MATING-TYPE PROTEIN A-1, [U-15N; U-13C], 1.5 mM; acetate, [U-2H], 25 mM; KCl 100 mM; NaN3 0.01%; H2O 90%; D2O 10%
sample_2: MATING-TYPE PROTEIN A-1, [U-15N], 1.5 mM; acetate, [U-2H], 25 mM; KCl 100 mM; NaN3 0.01%; H2O 90%; D2O 10%
sample_3: MATING-TYPE PROTEIN A-1 1.5 mM; acetate, [U-2H], 25 mM; KCl 100 mM; NaN3 0.01%; H2O 90%; D2O 10%
sample_cond_1: pH: 4.5; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
| HNCA-J | not available | not available | sample_cond_1 |
| 2D NOESY | not available | not available | sample_cond_1 |
| DQF-COSY | not available | not available | sample_cond_1 |
Software:
XWINNMR v2.3 - collection
FELIX v970 - data analysis, processing
X-PLOR v3.851 - refinement
NMR spectrometers:
- Bruker Avance 500 MHz
Related Database Links:
| PDB | |
| DBJ | GAA21999 |
| EMBL | CAA42252 CAY78246 CAY78300 |
| GB | AHY79765 AJP37516 AJQ32001 AJQ32146 AJQ32291 |
| REF | NP_010021 |
| SP | P0CY10 P0CY11 |
| TPG | DAA07567 |
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