BMRB Entry 4643
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4643
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Title: The solution structure of sheep myeloid antimicrobial peptide (smap29) and its relationship to biological function PubMed: 11856344
Deposition date: 2000-09-07 Original release date: 2002-09-23
Authors: Waring, A.; Sawai, M.; McCray, P.; Robertson, A.; Lehrer, R.
Citation: Tack, B.; Sawai, M.; Kearny, W.; Robertson, A.; Sherman, M.; Wang, W.; Hong, T.; Boo, L.; Wu, H.; Waring, A.; Lehrer, R.. "SMAP-29 has two LPS-binding sites and a central hinge" Eur. J. Biochem. 269, 1181-1189 (2002).
Assembly members:
MYELOID ANTIMICROBIAL PEPTIDE, polymer, 29 residues, Formula weight is not available
Natural source: Common Name: sheep Taxonomy ID: 9940 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Ovis aries
Experimental source: Production method: .
Entity Sequences (FASTA):
MYELOID ANTIMICROBIAL PEPTIDE: RGLRRLGRKIAHGVKKYGPT
VLRIIRIAG
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 203 |
| coupling constants | 8 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MYELOID ANTIMICROBIAL PEPTIDE | 1 |
Entities:
Entity 1, MYELOID ANTIMICROBIAL PEPTIDE 29 residues - Formula weight is not available
| 1 | ARG | GLY | LEU | ARG | ARG | LEU | GLY | ARG | LYS | ILE | ||||
| 2 | ALA | HIS | GLY | VAL | LYS | LYS | TYR | GLY | PRO | THR | ||||
| 3 | VAL | LEU | ARG | ILE | ILE | ARG | ILE | ALA | GLY |
Samples:
sample_1: MYELOID ANTIMICROBIAL PEPTIDE 1 mM; phosphate buffer 50 mM; H2O 60%; trifluoroethanol, [U-2H], 40%
sample_cond_1: pH: 5.94; temperature: 298 K; ionic strength: 50 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| DQF-COSY | sample_1 | not available | sample_cond_1 |
| TOCSY | sample_1 | not available | sample_cond_1 |
Software:
DYANA v1.5 - structure solution
VNMR v4.3b - collection
VNMR v6.1b - data analysis, refinement
NMR spectrometers:
- Varian UNITY 500 MHz