BMRB Entry 4743
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4743
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Title: 1H Chemical Shift Assignments for fully reduced cytochrome c7 from Desulfuromonas acetoxidan
Deposition date: 2000-05-22 Original release date: 2000-07-13
Authors: Assfalg, M.; Banci, L.; Bertini, I.; Bruschi, M.; Giudici-Orticoni, M.; Turano, P.
Citation: Assfalg, M.; Banci, L.; Bertini, I.; Bruschi, M.; Giudici-Orticoni, M.; Turano, P.. "A proton-NMR investigation of the fully reduced cytochrome c7 from Desulfuromonas acetoxidans. Comparison between the reduced and the oxidized forms." Eur. J. Biochem. 266, 634-643 (1999).
Assembly members:
CYTOCHROME C7, polymer, 68 residues, 9500 Da.
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: Desulfuromonas acetoxidans Taxonomy ID: 891 Superkingdom: Eubacteria Kingdom: not available Genus/species: Desulfuromonas acetoxidans
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
CYTOCHROME C7: ADVVTYENKKGNVTFDHKAH
AEKLGCDACHEGTPAKIAID
KKSAHKDACKTCHKSNNGPT
KCGGCHIK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 449 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CYTOCHROME C7 | 1 |
| 2 | heme c, 1 | 2 |
| 3 | heme c, 2 | 2 |
| 4 | heme c, 3 | 2 |
Entities:
Entity 1, CYTOCHROME C7 68 residues - 9500 Da.
| 1 | ALA | ASP | VAL | VAL | THR | TYR | GLU | ASN | LYS | LYS | ||||
| 2 | GLY | ASN | VAL | THR | PHE | ASP | HIS | LYS | ALA | HIS | ||||
| 3 | ALA | GLU | LYS | LEU | GLY | CYS | ASP | ALA | CYS | HIS | ||||
| 4 | GLU | GLY | THR | PRO | ALA | LYS | ILE | ALA | ILE | ASP | ||||
| 5 | LYS | LYS | SER | ALA | HIS | LYS | ASP | ALA | CYS | LYS | ||||
| 6 | THR | CYS | HIS | LYS | SER | ASN | ASN | GLY | PRO | THR | ||||
| 7 | LYS | CYS | GLY | GLY | CYS | HIS | ILE | LYS |
Entity 2, heme c, 1 - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
sample_1: CYTOCHROME C72 – 3 mM; sodium dithionite mM
sample_2: CYTOCHROME C72 – 3 mM
sample_cond_1: pH: 6.5; temperature: 292 K
sample_cond_2: pH: 6.5; temperature: 292 K
sample_cond_3: pH: 8.0; temperature: 292 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
Software:
AMBER v4.1 - refinement
DYANA v1.5 - structure solution
XEASY v3.1 - data analysis
XWINNMR - processing
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker Avance 800 MHz