BMRB Entry 4829
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4829
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Title: 1H, 15N and 13C resonance assignments for the DNA-binding domain of interleukin enhancer binding factor PubMed: 12402362
Deposition date: 2000-09-14 Original release date: 2002-11-14
Authors: Liu, Pei-Phen; Chen, Y.C.; Li, Chin; Hsieh, Yu-Huei; Chen, Shu-Wan; Chen, Shu-Huei; Jeng, W.Y.; Chuang, Woei-Jer
Citation: Liu, Pei-Phen; Chen, Y.C.; Li, Chin; Hsieh, Yu-Huei; Chen, Shu-Wan; Chen, Shu-Huei; Jeng, W.Y.; Chuang, Woei-Jer. "Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a)" Proteins: Struct., Funct., Genet. 49, 543-553 (2002).
Assembly members:
Interleukin enhancer binding factor, polymer, 98 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Interleukin enhancer binding factor: DSKPPYSYAQLIVQAITMAP
DKQLTLNGIYTHITKNYPYY
RTADKGWQNSIRHNLSLNRY
FIKVPRSQEEPGKGSFWRID
PASESKLIEQAFRKRRPR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 612 |
| 13C chemical shifts | 298 |
| 15N chemical shifts | 105 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ILF | 1 |
Entities:
Entity 1, ILF 98 residues - Formula weight is not available
| 1 | ASP | SER | LYS | PRO | PRO | TYR | SER | TYR | ALA | GLN | ||||
| 2 | LEU | ILE | VAL | GLN | ALA | ILE | THR | MET | ALA | PRO | ||||
| 3 | ASP | LYS | GLN | LEU | THR | LEU | ASN | GLY | ILE | TYR | ||||
| 4 | THR | HIS | ILE | THR | LYS | ASN | TYR | PRO | TYR | TYR | ||||
| 5 | ARG | THR | ALA | ASP | LYS | GLY | TRP | GLN | ASN | SER | ||||
| 6 | ILE | ARG | HIS | ASN | LEU | SER | LEU | ASN | ARG | TYR | ||||
| 7 | PHE | ILE | LYS | VAL | PRO | ARG | SER | GLN | GLU | GLU | ||||
| 8 | PRO | GLY | LYS | GLY | SER | PHE | TRP | ARG | ILE | ASP | ||||
| 9 | PRO | ALA | SER | GLU | SER | LYS | LEU | ILE | GLU | GLN | ||||
| 10 | ALA | PHE | ARG | LYS | ARG | ARG | PRO | ARG |
Samples:
sample_1: Interleukin enhancer binding factor, [U-13C; U-15N], 2.5 mM
sample_2: Interleukin enhancer binding factor, [U-15N], 2.5 mM
sample_3: Interleukin enhancer binding factor 2.5 mM
Cond_set_1: pH: 6.0; temperature: 300 K; ionic strength: 0.25 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HBHANH | not available | not available | not available |
| HBHA(CO)NH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| 1H-15N-TOCSY-HSQC | not available | not available | not available |
| 1H-15N-NOESY-HSQC | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| 2D COSY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE29561 |
| EMBL | CAA43200 CAG00839 |
| GB | AAB02820 AAB02821 AAH46369 AAI12950 AAI52768 |
| PIR | B42827 |
| REF | NP_001074401 NP_001100545 NP_001135634 NP_004505 XP_001114082 |
| SP | Q01167 Q3UCQ1 Q7ZX03 |
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