BMRB Entry 4900
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4900
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Title: 1H and 15N chemical shift assignments of the C-terminal xylan binding module of C. fimi xylanase 11A PubMed: 11327868
Deposition date: 2000-11-20 Original release date: 2001-08-08
Authors: Bolam, David; Xie, Hefang; White, Peter; Simpson, Peter; Hancock, Susan; Williamson, Michael; Gilbert, Harry
Citation: Bolam, David; Xie, Hefang; White, Peter; Simpson, Peter; Hancock, Susan; Williamson, Michael; Gilbert, Harry. "Evidence for Synergy Between Family 2b Carbohydrate Binding Modules in Cellulomonas fimi xylanase 11A" Biochemistry 40, 2468-2477 (2001).
Assembly members:
xylanase 11A, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 1708 Superkingdom: Eubacteria Kingdom: not available Genus/species: Cellulomonas fimi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
xylanase 11A: TGSCSVSAVRGEEWADRFNV
TYSVSGSSSWVVTLGLNGGQ
SVQSSWNAALTGSSGTVTAR
PNGSGNSFGVTFYKNGSSAT
PGATCATG
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 97 |
| 1H chemical shifts | 525 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | XBD | 1 |
Entities:
Entity 1, XBD 88 residues - Formula weight is not available
| 1 | THR | GLY | SER | CYS | SER | VAL | SER | ALA | VAL | ARG | ||||
| 2 | GLY | GLU | GLU | TRP | ALA | ASP | ARG | PHE | ASN | VAL | ||||
| 3 | THR | TYR | SER | VAL | SER | GLY | SER | SER | SER | TRP | ||||
| 4 | VAL | VAL | THR | LEU | GLY | LEU | ASN | GLY | GLY | GLN | ||||
| 5 | SER | VAL | GLN | SER | SER | TRP | ASN | ALA | ALA | LEU | ||||
| 6 | THR | GLY | SER | SER | GLY | THR | VAL | THR | ALA | ARG | ||||
| 7 | PRO | ASN | GLY | SER | GLY | ASN | SER | PHE | GLY | VAL | ||||
| 8 | THR | PHE | TYR | LYS | ASN | GLY | SER | SER | ALA | THR | ||||
| 9 | PRO | GLY | ALA | THR | CYS | ALA | THR | GLY |
Samples:
sample_1: xylanase 11A 1.0 mM
sample_2: xylanase 11A, [U-99% 15N], 1.0 mM
Ex-cond_1: pH: 5.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
| E.COSY | not available | not available | not available |
Software:
FELIX v97 -
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 500 MHz
Related Database Links:
| PDB |
Download simulated HSQC data in one of the following formats:
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SPARKY: Backbone
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