BMRB Entry 4910
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4910
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Title: 1H and 15N chemical shift assignments for LEKTI domain one (HF6478) PubMed: 12684009
Deposition date: 2000-12-06 Original release date: 2005-11-11
Authors: Lauber, Thomas; Adermann, Knut; Schulz, Axel; Forssmann, Wolf; Roesch, Paul; Marx, Ute
Citation: Lauber, Thomas; Schulz, Axel; Schweimer, K.; Adermann, Knut; Marx, Ute. "Homologous proteins with different folds: the three-dimensional structures of domains 1 and 6 of the multiple Kazal-type inhibitor LEKTI" J. Mol. Biol. 328, 205-219 (2003).
Assembly members:
HF6478, polymer, 55 residues, 6478 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
HF6478: KNEDQEMCHEFQAFMKNGKL
FCPQDKKFFQSLDGIMFINK
CATCKMILEKEAKSQ
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 15N chemical shifts | 51 |
| 1H chemical shifts | 686 |
| coupling constants | 13 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HF6478 | 1 |
Entities:
Entity 1, HF6478 55 residues - 6478 Da.
| 1 | LYS | ASN | GLU | ASP | GLN | GLU | MET | CYS | HIS | GLU | ||||
| 2 | PHE | GLN | ALA | PHE | MET | LYS | ASN | GLY | LYS | LEU | ||||
| 3 | PHE | CYS | PRO | GLN | ASP | LYS | LYS | PHE | PHE | GLN | ||||
| 4 | SER | LEU | ASP | GLY | ILE | MET | PHE | ILE | ASN | LYS | ||||
| 5 | CYS | ALA | THR | CYS | LYS | MET | ILE | LEU | GLU | LYS | ||||
| 6 | GLU | ALA | LYS | SER | GLN |
Samples:
sample_1: HF6478, [U-15N], 1.5 mM
sample_2: HF6478 1.2 mM
Ex-cond_1: pH: 4.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D-TOCSY | not available | not available | not available |
| 2D-NOESY | not available | not available | not available |
| 2D-COSY | not available | not available | not available |
| 1H-15N-HSQC | not available | not available | not available |
| HNHA | not available | not available | not available |
| 3D-1H-15N-TOCSY-HSQC | not available | not available | not available |
| 3D-1H-15N-NOESY-HSQC | not available | not available | not available |
| 3D-1H-15N/1H-15N-HMQC-NOESY-HSQC | not available | not available | not available |
Software:
NDEE, Spinup Inc. -
NMRView v4.1.0 -
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB |
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