BMRB Entry 4921
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4921
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Title: Solution structure of poneratoxin PubMed: 15153103
Deposition date: 2000-12-13 Original release date: 2008-07-16
Authors: Poznanski, J.
Citation: Szolajska, E.; Poznanski, J.; Ferber, M.; Michalik, J.; Gout, E.; Fender, P.; Bailly, I.; Dublet, B.; Chroboczek, J.. "Poneratoxin, a neurotoxin from ant venom. Structure and expression in insect cells and construction of a bio-insecticide" Eur. J. Biochem. 271, 2127-2136 (2004).
Assembly members:
Poneratoxin, polymer, 26 residues, Formula weight is not available
Natural source: Common Name: ant Taxonomy ID: 55425 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Paraponera clavata
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Poneratoxin: FLPLLILGSLLMTPPVIQAI
HDAQRX
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 153 |
| coupling constants | 20 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Poneratoxin | 1 |
Entities:
Entity 1, Poneratoxin 26 residues - Formula weight is not available
| 1 | PHE | LEU | PRO | LEU | LEU | ILE | LEU | GLY | SER | LEU | ||||
| 2 | LEU | MET | THR | PRO | PRO | VAL | ILE | GLN | ALA | ILE | ||||
| 3 | HIS | ASP | ALA | GLN | ARG | NH2 |
Samples:
sample: Poneratoxin 3 mM; D2O 75%; TFE 25%
cond: pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | sample | not available | cond |
| 2D NOESY | sample | not available | cond |
| TOCSY | sample | not available | cond |
Software:
UXNMR v930601.3 - data collection
NMRPipe v1.7 - data processing
XEASY v1.3.11 - data analysis
DYANA v1.5 - structure solution
X-PLOR v3.1 - refinement
SYBYL v6.5 - data analysis
NMR spectrometers:
- Bruker AMX 600 MHz