BMRB Entry 4933
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4933
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Title: 1H and 15N Chemical Shift Assignments for a Cys58Ser mutant of TFF1
Deposition date: 2000-12-22 Original release date: 2001-02-15
Authors: Williams, Mark; Polshakov, Vladimir; Feeney, James
Citation: Polshakov, Vladimir; Thomas, Frenkiel; Westley, Bruce; Chadwick, Mark; May, Felicity; Carr, Mark; Feeney, James. "NMR-based structural Studies of the pNR-2/pS2 single domain trefoil peptide" Eur. J. Biochem. 233, 847-855 (1995).
Assembly members:
Trefoil Factor Family 1, polymer, 60 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Trefoil Factor Family 1: EAQTETCTVAPRERQNCGFP
GVTPSQCANKGCCFDDTVRG
VPWCFYPNTIDVPPEEESEF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 386 |
| 15N chemical shifts | 59 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TFF1 mutant | 1 |
Entities:
Entity 1, TFF1 mutant 60 residues - Formula weight is not available
| 1 | GLU | ALA | GLN | THR | GLU | THR | CYS | THR | VAL | ALA | |
| 2 | PRO | ARG | GLU | ARG | GLN | ASN | CYS | GLY | PHE | PRO | |
| 3 | GLY | VAL | THR | PRO | SER | GLN | CYS | ALA | ASN | LYS | |
| 4 | GLY | CYS | CYS | PHE | ASP | ASP | THR | VAL | ARG | GLY | |
| 5 | VAL | PRO | TRP | CYS | PHE | TYR | PRO | ASN | THR | ILE | |
| 6 | ASP | VAL | PRO | PRO | GLU | GLU | GLU | SER | GLU | PHE |
Samples:
sample_1: Trefoil Factor Family 1 1.8 mM; sodium phosphate 5 mM
sample_2: Trefoil Factor Family 1, [U-95% 15N], 1.3 mM; sodium phosphate 5 mM
cond_1: pH: 5.8; temperature: 298 K; ionic strength: 0.01 M
cond_2: pH: 5.8; temperature: 283 K; ionic strength: 0.01 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| ROESY | not available | not available | not available |
| 13C-1H HSQC | not available | not available | not available |
| 13C-1H HSQC-TOCSY | not available | not available | not available |
| 15N-1H HSQC | not available | not available | not available |
| 15N-1H HSQC-NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian UNITY 600 MHz
- Varian UNITY plus 500 MHz
Related Database Links:
| BMRB | 4930 |
| PDB | |
| DBJ | BAA95532 BAB13729 |
| EMBL | CAA25155 CAA28695 CAA36254 |
| GB | AAA52402 AAH32811 AAX36234 AAX36702 AAX41076 |
| PRF | 1502207A 1716375A |
| REF | NP_003216 XP_008975839 XP_009433935 |
| SP | P04155 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts