BMRB Entry 4939
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4939
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Title: Solution Structures of Two CCHC Zinc Fingers from the FOG Family Protein U-shaped that Mediate Protein-Protein Interactions
Deposition date: 2001-01-13 Original release date: 2001-01-23
Authors: Kowalski, K.; Mackay, J.
Citation: Liew, C.; Kowalski, K.; Fox, A.; Newton, A.; Sharpe, B.; Crossley, M.; Mackay, J.. "Solution Structures of Two CCHC Zinc Fingers from the FOG Family Protein U-shaped that Mediate Protein-Protein Interactions" Structure 8, 1157-1166 (2000).
Assembly members:
U-shaped transcriptional cofactor, polymer, 36 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
U-shaped transcriptional cofactor: GSAAEVMKKYCSTCDISFNY
VKTYLAHKQFYCKNKP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 210 |
| 15N chemical shifts | 34 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | U-shaped transcriptional cofactor | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, U-shaped transcriptional cofactor 36 residues - Formula weight is not available
| 1 | GLY | SER | ALA | ALA | GLU | VAL | MET | LYS | LYS | TYR | ||||
| 2 | CYS | SER | THR | CYS | ASP | ILE | SER | PHE | ASN | TYR | ||||
| 3 | VAL | LYS | THR | TYR | LEU | ALA | HIS | LYS | GLN | PHE | ||||
| 4 | TYR | CYS | LYS | ASN | LYS | PRO |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: U-shaped transcriptional cofactor 0.4 mM; ZnSO4 0.6 mM; TCEP 0.6 mM
sample_2: U-shaped transcriptional cofactor, [U-15N], 0.2 mM; ZnSO4 0.3 mM; TCEP 0.3 mM
sample_cond_1: pH: 5.0; temperature: 293 K; ionic strength: 0.6 mM; pressure: 1 atm
sample_cond_2: pH: 5.0; temperature: 293 K; ionic strength: 0.3 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
Software:
XWINNMR v2.1 - data processing
XEASY v1.3.13 - data analysis
DYANA v1.5 - structure solution
CNS v0.5 - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts