BMRB Entry 4972
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4972
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Title: 1H, 13C, and 15N assignment of Crh, a protein involved in carbon catabolite repression in B. subtilis PubMed: 11916384
Deposition date: 2001-03-14 Original release date: 2003-04-09
Authors: Favier, Adrien; Brutscher, Bernhard; Blackledge, M.; Galinier, Anne; Deutscher, J.; Penin, Francois; Marion, Dominique
Citation: Favier, Adrien; Brutscher, Bernhard; Blackledge, M.; Galinier, Anne; Deutscher, J.; Penin, Francois; Marion, Dominique. "Solution structure and dynamics of Crh, the Bacillus subtilis catabolite repression HPr" J. Mol. Biol. 317, 131-144 (2002).
Assembly members:
catabolite repression HPr, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus subtilis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
catabolite repression HPr: MVQQKVEVRLKTGLQARPAA
LFVQEANRFTSDVFLEKDGK
KVNAKSIMGLMSLAVSTGTE
VTLIAQGEDEQEALEKLAAY
VQEEVLQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 604 |
| 13C chemical shifts | 354 |
| 15N chemical shifts | 91 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Crh | 1 |
Entities:
Entity 1, Crh 87 residues - Formula weight is not available
| 1 | MET | VAL | GLN | GLN | LYS | VAL | GLU | VAL | ARG | LEU | ||||
| 2 | LYS | THR | GLY | LEU | GLN | ALA | ARG | PRO | ALA | ALA | ||||
| 3 | LEU | PHE | VAL | GLN | GLU | ALA | ASN | ARG | PHE | THR | ||||
| 4 | SER | ASP | VAL | PHE | LEU | GLU | LYS | ASP | GLY | LYS | ||||
| 5 | LYS | VAL | ASN | ALA | LYS | SER | ILE | MET | GLY | LEU | ||||
| 6 | MET | SER | LEU | ALA | VAL | SER | THR | GLY | THR | GLU | ||||
| 7 | VAL | THR | LEU | ILE | ALA | GLN | GLY | GLU | ASP | GLU | ||||
| 8 | GLN | GLU | ALA | LEU | GLU | LYS | LEU | ALA | ALA | TYR | ||||
| 9 | VAL | GLN | GLU | GLU | VAL | LEU | GLN |
Samples:
sample_1: catabolite repression HPr, [U-95% 13C; U-90% 15N], 0.4 – 0.5 mM
sample_2: catabolite repression HPr, [U-90% 15N], 0.4 – 0.5 mM
Ex-cond_1: pH: 7.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-edited NOESY-HSQC | not available | not available | not available |
| 3D 15N-edited TOCSY-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| H(C)C(CO)NH-TOCSY | not available | not available | not available |
| (H)C(CO)NH-TOCSY | not available | not available | not available |
| H(C)CH-TOCSY | not available | not available | not available |
Software:
FELIX v2000 -
NMR spectrometers:
- VARIAN INOVA 600 MHz
- VARIAN INOVA 800 MHz
Related Database Links:
| BMRB | 5757 |
| PDB | |
| DBJ | BAI87096 BAM55550 BAM59563 GAK79281 |
| EMBL | CAB08060 CAB15479 CCU60557 CEI59278 CEJ79135 |
| GB | ADM39440 ADV94285 AEP88381 AEP92509 AFI30049 |
| REF | NP_391354 WP_003219835 WP_024122965 WP_024713407 YP_003867749 |
| SP | O06976 |
Download simulated HSQC data in one of the following formats:
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