BMRB Entry 5065

Name: Quail Cysteine and Glycine-rich Protein, NMR, 15 Minimized Model Structures
Published: 2001-11-14

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5065

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Quail Cysteine and Glycine-rich Protein, NMR, 15 Minimized Model Structures PubMed: 11583159

Deposition date: 2001-06-29 Original release date: 2001-11-14

Authors: Schuler, Wolfgang; Kloiber, Karin; Matt, Theresia; Bister, Klaus; Konrat, Robert

Citation: Schuler, Wolfgang; Kloiber, Karin; Matt, Theresia; Bister, Klaus; Konrat, Robert. "Application of Cross-correlated NMR Spin Relaxation to the Zinc-finger Protein CRP2(LIM2): Evidence for Collective Motions in LIM Domains" Biochemistry 40, 9596-9604 (2001).

Assembly members:
CYSTEINE-RICH PROTEIN CRP2(LIM2), polymer, 113 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source: Common Name: Japanese Quail Taxonomy ID: 93934 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Coturnix japonica

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):
CYSTEINE-RICH PROTEIN CRP2(LIM2): MDRGERLGIKPESSPSPHRP TTNPNTSKFAQKFGGAEKCS RCGDSVYAAEKVIGAGKPWH KNCFRCAKCGKSLESTTLTE KEGEIYCKGCYAKNFGPKGF GYGQGAGALVHAQ

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	231
15N chemical shifts	56
1H chemical shifts	336

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CYSTEINE-RICH PROTEIN	1
2	ZINC ION, I	2
3	ZINC ION, II	2

Entities:

Entity 1, CYSTEINE-RICH PROTEIN 113 residues - Formula weight is not available

1

MET

ASP

ARG

GLY

GLU

ARG

LEU

GLY

ILE

LYS

2

PRO

GLU

SER

PRO

SER

PRO

HIS

ARG

PRO

3

THR

ASN

PRO

ASN

THR

SER

LYS

PHE

ALA

4

GLN

LYS

PHE

GLY

ALA

GLU

LYS

CYS

SER

5

ARG

CYS

GLY

ASP

SER

VAL

TYR

ALA

GLU

6

LYS

VAL

ILE

GLY

ALA

GLY

LYS

PRO

TRP

HIS

7

LYS

ASN

CYS

PHE

ARG

CYS

ALA

LYS

CYS

GLY

8

LYS

SER

LEU

GLU

SER

THR

LEU

THR

GLU

9

LYS

GLU

GLY

GLU

ILE

TYR

CYS

LYS

GLY

CYS

10

TYR

ALA

LYS

ASN

PHE

GLY

PRO

LYS

GLY

PHE

11

GLY

TYR

GLY

GLN

GLY

ALA

GLY

ALA

LEU

VAL

12

HIS

ALA

GLN

Entity 2, ZINC ION, I - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: CYSTEINE-RICH PROTEIN CRP2(LIM2), [U-13C; U-15N], 1.0 – 2.0 mM; H2O 90%; D2O, [U-2H], 10%; potassium phosphate 20 mM; KCl 50 mM; dithiothreitol 0.5 mM

sample_2: CYSTEINE-RICH PROTEIN CRP2(LIM2), [U-15N], 1.0 – 2.0 mM; H2O 90%; D2O, [U-2H], 10%; potassium phosphate 20 mM; KCl 50 mM; dithiothreitol 0.5 mM

sample_cond_1: ionic strength: 70.5 mM; pH: 7.2; pressure: 1 atm; temperature: 299 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-HSQC	not available	not available	sample_cond_1
3D HNCA	not available	not available	sample_cond_1
3D HNCACB	not available	not available	sample_cond_1
3D HNCO	not available	not available	sample_cond_1
3D CBCA(CO)NH	not available	not available	sample_cond_1
3D HCCH-TOCSY	not available	not available	sample_cond_1
3D CCH-TOCSY-NNH	not available	not available	sample_cond_1
3D 13C-NOESY-HSQC (CA-centered)	not available	not available	sample_cond_1
3D 13C-NOESY-HSQC (methyl-centered)	not available	not available	sample_cond_1
3D 15N-NOESY-HSQC	not available	not available	sample_cond_1

Software:

VNMR vv6.1 Rev.B - collection

NMRPipe vv1.8 Rev 2001.030.21.27 - processing

ANSIG vv3.3 - data analysis

CNS v1.0 - structure solution

X-PLOR v3.851 - refinement

NMR spectrometers:

VARIAN UNITYPLUS 500 MHz

Biological Magnetic Resonance Data Bank