BMRB Entry 5073
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5073
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Title: Solution structure of the monomeric variant of the chemokine MIP-1beta PubMed: 11535053
Deposition date: 2001-07-09 Original release date: 2003-01-07
Authors: Kim, S.; Jao, S.; Laurence, J.; LiWang, P.
Citation: Kim, S.; Jao, S.; Laurence, J.; LiWang, P.. "Structural Comparison of Monomeric Variants of the Chemokine MIP-1beta having Differing Ability to bind the Receptor CCR5" Biochemistry 40, 10782-10791 (2001).
Assembly members:
MIP-1 beta, polymer, 69 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
MIP-1 beta: APMGSDPPTACCASYTARKL
PRNFVVDYYETSSLCSQPAV
VFQTKRSKQVCADPSESWVQ
EYVYDLELN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 190 |
| 15N chemical shifts | 62 |
| 1H chemical shifts | 376 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MIP-1-beta | 1 |
Entities:
Entity 1, MIP-1-beta 69 residues - Formula weight is not available
| 1 | ALA | PRO | MET | GLY | SER | ASP | PRO | PRO | THR | ALA | ||||
| 2 | CYS | CYS | ALA | SER | TYR | THR | ALA | ARG | LYS | LEU | ||||
| 3 | PRO | ARG | ASN | PHE | VAL | VAL | ASP | TYR | TYR | GLU | ||||
| 4 | THR | SER | SER | LEU | CYS | SER | GLN | PRO | ALA | VAL | ||||
| 5 | VAL | PHE | GLN | THR | LYS | ARG | SER | LYS | GLN | VAL | ||||
| 6 | CYS | ALA | ASP | PRO | SER | GLU | SER | TRP | VAL | GLN | ||||
| 7 | GLU | TYR | VAL | TYR | ASP | LEU | GLU | LEU | ASN |
Samples:
sample_1: MIP-1 beta, [U-15N; U-13C], 1 – 2 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 20 mM; pH: 2.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| HNHA | not available | not available | not available |
Software:
NMRPipe - processing
CNS v1.0 - structure solution
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| EMBL | CAA34291 CAA37723 CAG46916 |
| GenBank | AAA51576 AAA57256 AAI07434 AAX07292 AAX07305 |
| REF | NP_002975 |
| SWISS-PROT | P13236 |
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