BMRB Entry 5074
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5074
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Title: LEM domain of human inner nuclear membrane protein emerin PubMed: 11470279
Deposition date: 2001-07-09 Original release date: 2003-01-07
Authors: Wolff, N.; Gilquin, B.; Courchay, K.; Callebaut, I.; Worman, H.; Zinn-Justin, S.
Citation: Wolff, N.; Gilquin, B.; Courchay, K.; Callebaut, I.; Worman, H.; Zinn-Justin, S.. "Structural Analysis of Emerin, an inner Nuclear Membrane Protein Mutated in X-linked Emery-Dreifuss Muscular Dystrophy " FEBS Lett. 501, 171-176 (2001).
Assembly members:
EMERIN, polymer, 53 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
EMERIN: DNYADLSDTELTTLLRRYNI
PHGPVVGSTRRLYEKKIFEY
ETQRRRLSPPSSS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 345 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EMERIN | 1 |
Entities:
Entity 1, EMERIN 53 residues - Formula weight is not available
| 1 | ASP | ASN | TYR | ALA | ASP | LEU | SER | ASP | THR | GLU | ||||
| 2 | LEU | THR | THR | LEU | LEU | ARG | ARG | TYR | ASN | ILE | ||||
| 3 | PRO | HIS | GLY | PRO | VAL | VAL | GLY | SER | THR | ARG | ||||
| 4 | ARG | LEU | TYR | GLU | LYS | LYS | ILE | PHE | GLU | TYR | ||||
| 5 | GLU | THR | GLN | ARG | ARG | ARG | LEU | SER | PRO | PRO | ||||
| 6 | SER | SER | SER |
Samples:
sample_1: EMERIN 1 mM; NaH2PO4/Na2HPO4 20 mM; H2O 90%; D2O 10%
sample_cond_1: pH: 6.3; temperature: 298 K; ionic strength: 20 mM
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
Software:
FELIX v2.0 - data analysis
X-PLOR v3.1 - structure solution
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA10972 BAG73116 |
| EMBL | CAA57817 CAA60500 CAG38773 CAL38599 |
| GB | AAA92645 AAH00738 AAP36065 AAP36933 AAX32283 |
| REF | NP_000108 XP_003279349 XP_003779813 XP_003804767 XP_004065169 |
| SP | P50402 |