BMRB Entry 5077
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5077
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Title: 1H, 15N, 13C NMR Assignments of M156R Protein from myxoma virus, NESG target OP2 PubMed: 12367520
Deposition date: 2001-07-11 Original release date: 2002-10-10
Authors: Ramelot, Theresa; Cort, John; Yee, Adelinda; Furong, L.; Goshe, M.; Edwards, Aled; Smith, R.; Arrowsmith, Cheryl; Dever, T.; Kennedy, Michael
Citation: Ramelot, Theresa; Cort, John; Yee, Adelinda; Furong, L.; Goshe, M.; Edwards, Aled; Smith, R.; Arrowsmith, Cheryl; Dever, T.; Kennedy, Michael. "Myxoma virus immunomodulatory protein M156R is a structural mimic of eukaryotic translation initiation factor eIF2a" J. Mol. Biol. 322, 943-954 (2002).
Assembly members:
M156R, polymer, 102 residues, 11974 Da.
Natural source: Common Name: Myxoma virus Taxonomy ID: 10273 Superkingdom: Viruses Kingdom: not available Genus/species: Leporipoxvirus Poxvirus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
M156R: MTVIKPSSRPRPRKNKNIKV
NTYRTSAMDLSPGSVHEGIV
YFKDGIFKVRLLGYEGHECI
LLDYLNYRQDTLDRLKERLV
GRVIKTRVVRADGLYVDLRR
FF
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 563 |
| 13C chemical shifts | 374 |
| 15N chemical shifts | 83 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | m156r | 1 |
Entities:
Entity 1, m156r 102 residues - 11974 Da.
| 1 | MET | THR | VAL | ILE | LYS | PRO | SER | SER | ARG | PRO | ||||
| 2 | ARG | PRO | ARG | LYS | ASN | LYS | ASN | ILE | LYS | VAL | ||||
| 3 | ASN | THR | TYR | ARG | THR | SER | ALA | MET | ASP | LEU | ||||
| 4 | SER | PRO | GLY | SER | VAL | HIS | GLU | GLY | ILE | VAL | ||||
| 5 | TYR | PHE | LYS | ASP | GLY | ILE | PHE | LYS | VAL | ARG | ||||
| 6 | LEU | LEU | GLY | TYR | GLU | GLY | HIS | GLU | CYS | ILE | ||||
| 7 | LEU | LEU | ASP | TYR | LEU | ASN | TYR | ARG | GLN | ASP | ||||
| 8 | THR | LEU | ASP | ARG | LEU | LYS | GLU | ARG | LEU | VAL | ||||
| 9 | GLY | ARG | VAL | ILE | LYS | THR | ARG | VAL | VAL | ARG | ||||
| 10 | ALA | ASP | GLY | LEU | TYR | VAL | ASP | LEU | ARG | ARG | ||||
| 11 | PHE | PHE |
Samples:
sample_1: M156R, [U-15N; U-13C], 1 mM; phosphate buffer 25 mM; NaCl 450 mM
sample_2: M156R, [U-15N], 1 mM; phosphate buffer 25 mM; NaCl 450 mM
cond_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| 15N-edited NOESY-HQSC | not available | not available | not available |
| HCC-TOCSY-NNH | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| CN-NOESY-HSQC | not available | not available | not available |
| CBCACOCAHA | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| CCC-TOCSY-NNH | not available | not available | not available |
| HNHA | not available | not available | not available |
| 4D CC-NOESY | not available | not available | not available |
Software:
Felix v98 - processing data
XPLOR v3.84 - structure calculations
NMR spectrometers:
- Varian Inova 500 MHz
- Varian Inova 600 MHz
- Varian Inova 750 MHz
- Varian Inova 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts