BMRB Entry 5086
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5086
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Title: Reduced recombinant Hydrogenobacter thermophilus cytochrome c-552 expressed in E. coli periplasm PubMed: 11935350
Deposition date: 2001-07-23 Original release date: 2002-08-22
Authors: Karan, Elizabeth; Russell, Brandy; Bren, Kara
Citation: Karan, Elizabeth; Russell, Brandy; Bren, Kara. "Characterization of Hydrogenobacter thermophilus Cytochromes c(552) Expressed in the Cytoplasm and Periplasm of Escherichia coli" J. Biol. Inorg. Chem. 7, 260-272 (2002).
Assembly members:
cytochrome c-552, polymer, 80 residues, 9187 Da.
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: Hydrogenobacter thermophilus Taxonomy ID: 940 Superkingdom: Bacteria Kingdom: not available Genus/species: Hydrogenobacter thermophilus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
cytochrome c-552: NEQLAKQKGCMACHDLKAKK
VGPAYADVAKKYAGRKDAVD
YLAGKIKKGGSGVWGSVPMP
PQNVTDAEAKQLAQWILSIK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 524 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ht p-cyt c-552 | 1 |
| 2 | heme | 2 |
Entities:
Entity 1, Ht p-cyt c-552 80 residues - 9187 Da.
| 1 | ASN | GLU | GLN | LEU | ALA | LYS | GLN | LYS | GLY | CYS | |
| 2 | MET | ALA | CYS | HIS | ASP | LEU | LYS | ALA | LYS | LYS | |
| 3 | VAL | GLY | PRO | ALA | TYR | ALA | ASP | VAL | ALA | LYS | |
| 4 | LYS | TYR | ALA | GLY | ARG | LYS | ASP | ALA | VAL | ASP | |
| 5 | TYR | LEU | ALA | GLY | LYS | ILE | LYS | LYS | GLY | GLY | |
| 6 | SER | GLY | VAL | TRP | GLY | SER | VAL | PRO | MET | PRO | |
| 7 | PRO | GLN | ASN | VAL | THR | ASP | ALA | GLU | ALA | LYS | |
| 8 | GLN | LEU | ALA | GLN | TRP | ILE | LEU | SER | ILE | LYS |
Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
sample_1: cytochrome c-5522 – 3.5 mM; heme c2 – 3.5 mM; sodium acetate, [U-2H], 120 mM; sodium dithionite mM
condition_1: pH: 5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H TOCSY | sample_1 | not available | condition_1 |
| 1H NOESY | sample_1 | not available | condition_1 |
Software:
FELIX v97 -
NMR spectrometers:
- Varian INOVA 500 MHz