BMRB Entry 5092
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5092
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Title: 1H chemical shift assignments for the cytoplasmic N-terminus of KCNMB2 PubMed: 11517232
Deposition date: 2001-07-26 Original release date: 2001-07-31
Authors: Bentrop, Detlef; Beyermann, Michael; Wissmann, Ralph; Fakler, Bernd
Citation: Bentrop, Detlef; Beyermann, Michael; Wissmann, Ralph; Fakler, Bernd. "NMR Structure of the "Ball-and-chain" Domain of KCNMB2, the beta 2-subunit of large Conductance Ca2+ and Voltage-activated Potassium Channels" J. Biol. Chem. 276, 42116-41121 (2001).
Assembly members:
beta2 subunit of BK channels, polymer, 45 residues, 5367 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
beta2 subunit of BK channels: MFIWTSGRTSSSYRHDEKRN
IYQKIRDHDLLDKRKTVTAL
KAGED
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 324 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | N-terminus of KCNMB2 | 1 |
Entities:
Entity 1, N-terminus of KCNMB2 45 residues - 5367 Da.
| 1 | MET | PHE | ILE | TRP | THR | SER | GLY | ARG | THR | SER | ||||
| 2 | SER | SER | TYR | ARG | HIS | ASP | GLU | LYS | ARG | ASN | ||||
| 3 | ILE | TYR | GLN | LYS | ILE | ARG | ASP | HIS | ASP | LEU | ||||
| 4 | LEU | ASP | LYS | ARG | LYS | THR | VAL | THR | ALA | LEU | ||||
| 5 | LYS | ALA | GLY | GLU | ASP |
Samples:
sample_1: beta2 subunit of BK channels 2.0 mM
conditions_1: pH: 3.0; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CLEAN-TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
Software:
XEASY -
NMR spectrometers:
- Bruker Avance 600 MHz