BMRB Entry 5096
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5096
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Title: Solution Structure of a CCHH mutant of the ninth CCHC Zinc Finger of U-shaped PubMed: 12110675
Deposition date: 2001-08-02 Original release date: 2003-06-02
Authors: Kowalski, K.; Mackay, J.
Citation: Kowalski, K.; Liew, C.; Matthews, J.; Gell, D.; Crossley, M.; Mackay, J.. "Characterization of the Conserved Interaction between GATA and FOG Family Proteins" J. Biol. Chem. 277, 35720-35729 (2002).
Assembly members:
U-shaped ninth zinc finger domain, polymer, 36 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
U-shaped ninth zinc finger domain: GSAAEVMKKYCSTCDISFNY
VKTYLAHKQFYHKNKP
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 208 |
| coupling constants | 24 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | U-shaped transcriptional cofactor | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, U-shaped transcriptional cofactor 36 residues - Formula weight is not available
| 1 | GLY | SER | ALA | ALA | GLU | VAL | MET | LYS | LYS | TYR | ||||
| 2 | CYS | SER | THR | CYS | ASP | ILE | SER | PHE | ASN | TYR | ||||
| 3 | VAL | LYS | THR | TYR | LEU | ALA | HIS | LYS | GLN | PHE | ||||
| 4 | TYR | HIS | LYS | ASN | LYS | PRO |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: U-shaped ninth zinc finger domain 1.0 mM; ZnSO4 1.5 mM; TCEP 1.5 mM
sample_2: U-shaped ninth zinc finger domain, [U-95% 15N], 0.5 mM; ZnSO4 0.75 mM; TCEP 0.75 mM
sample_cond_1: ionic strength: 1.5 mM; pH: 5.0; pressure: 1 atm; temperature: 293 K
sample_cond_2: ionic strength: 0.75 mM; pH: 5.0; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
| E-COSY | not available | not available | not available |
| HNHA | not available | not available | not available |
Software:
xwinnmr v2.6 - processing
DYANA v1.5 - structure solution
CNS v0.5 - refinement
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB |