BMRB Entry 5101
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5101
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Title: Structure and Properties of a Dimeric N-terminal Fragment of Human Ubiquitin PubMed: 117733996
Deposition date: 2001-08-06 Original release date: 2002-01-25
Authors: Bolton, David; Evans, Philip; Stott, Katherine; Broadhurst, Richard
Citation: Bolton, David; Evans, Philip; Stott, Katherine; Broadhurst, Richard. "Structure and Properties of a Dimeric N-terminal Fragment of Human Ubiquitin" J. Mol. Biol. 314, 773-787 (2001).
Assembly members:
Ubiquitin, polymer, 53 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Ubiquitin: GSMQIFVKTLTGKTITLEVE
PSDTIENVKAKIQDKEGIPP
DQQRLIFAGKQLE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 390 |
| 13C chemical shifts | 174 |
| 15N chemical shifts | 49 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | uq1_51 subunit A | 1 |
| 2 | uq1_51 subunit B | 1 |
Entities:
Entity 1, uq1_51 subunit A 53 residues - Formula weight is not available
| 1 | GLY | SER | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | ||||
| 2 | THR | GLY | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | ||||
| 3 | PRO | SER | ASP | THR | ILE | GLU | ASN | VAL | LYS | ALA | ||||
| 4 | LYS | ILE | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ||||
| 5 | ASP | GLN | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | ||||
| 6 | GLN | LEU | GLU |
Samples:
sample_1: Ubiquitin, [U-100% 13C; U-100% 15N], 1.0 mM; sodium sulphate 0.8 M
sample_2: Ubiquitin 1.0 mM; sodium sulphate 0.8 M
conditions_1: pH: 7.0 na; temperature: 298 K; ionic strength: 0.8 M; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-1H-15N NOESY | not available | not available | not available |
| 3D 1H-1H-15N TOCSY | not available | not available | not available |
| 3D 13C-1H-1H NOESY | not available | not available | not available |
| 3D 1H-13C-1H HCCH-TOCSY | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
Software:
AZARA - data processing
ANSIG v3.3 - assignment
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| BMRB | 11505 11547 15047 15410 15689 15866 15907 16228 16582 16626 16763 16880 16885 16895 17059 17181 17239 17333 17439 17769 17919 18582 18583 18584 18610 18611 18737 19394 19399 19406 19412 19447 25070 25230 4245 4375 5387 6457 6466 6470 6488 68 7111 |
| PDB | |
| DBJ | BAA03983 BAA11389 BAA11842 BAA11843 BAA23486 |
| EMBL | CAA28495 CAA30815 CAA33466 CAA35999 CAA37227 |
| GB | AAA02769 AAA28153 AAA28154 AAA28997 AAA28998 |
| PIR | I50437 I51568 I65237 JN0790 S13928 |
| PRF | 0412265A 1212243A 1212243B 1212243C 1212243D |
| REF | NP_001005123 NP_001006688 NP_001009117 NP_001009202 NP_001009286 |
| SP | P0C273 P0C275 P0C276 P0CG47 P0CG48 |
| TPG | DAA18802 DAA20663 DAA20672 DAA24675 DAA26453 |
Download simulated HSQC data in one of the following formats:
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