BMRB Entry 5175
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5175
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Title: Backbone 1H, 13C, 15N and sidechain 1H Chemical shift of CPI-17 PubMed: 11734001
Deposition date: 2001-10-11 Original release date: 2002-05-06
Authors: Ohki, Shin-ya; Eto, Masumi; Kariya, Eri; Hayano, Toshiya; Hayashi, Yuichiro; Yazawa, Michio; Brautigan, David; Kainosho, Masatsune
Citation: Ohki, Shin-ya; Eto, Masumi; Kariya, Eri; Hayano, Toshiya; Hayashi, Yuichiro; Yazawa, Michio; Brautigan, David; Kainosho, Masatsune. "Solution NMR Structure of the Myosin Phosphatase Inhibitor Protein CPI-17 Shows Phosphorylation-induced Conformational Changes Responsible for Activation " J. Mol. Biol. 314, 839-849 (2001).
Assembly members:
CPI-17, polymer, 86 residues, Formula weight is not available
Natural source: Common Name: 9823 Taxonomy ID: Pig Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Sus scrofa
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
CPI-17: ARVTVKYDRRELQRRLDVEK
WIDGRLEELYRGREADMPDE
VNIDELLELESEEERSRKIQ
GLLKSCTNPTENFVQELLVK
LRGLHK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 603 |
| 13C chemical shifts | 168 |
| 15N chemical shifts | 84 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CPI-17 | 1 |
Entities:
Entity 1, CPI-17 86 residues - Formula weight is not available
| 1 | ALA | ARG | VAL | THR | VAL | LYS | TYR | ASP | ARG | ARG | ||||
| 2 | GLU | LEU | GLN | ARG | ARG | LEU | ASP | VAL | GLU | LYS | ||||
| 3 | TRP | ILE | ASP | GLY | ARG | LEU | GLU | GLU | LEU | TYR | ||||
| 4 | ARG | GLY | ARG | GLU | ALA | ASP | MET | PRO | ASP | GLU | ||||
| 5 | VAL | ASN | ILE | ASP | GLU | LEU | LEU | GLU | LEU | GLU | ||||
| 6 | SER | GLU | GLU | GLU | ARG | SER | ARG | LYS | ILE | GLN | ||||
| 7 | GLY | LEU | LEU | LYS | SER | CYS | THR | ASN | PRO | THR | ||||
| 8 | GLU | ASN | PHE | VAL | GLN | GLU | LEU | LEU | VAL | LYS | ||||
| 9 | LEU | ARG | GLY | LEU | HIS | LYS |
Samples:
sample_1: CPI-170.8 – 1.3 mM
Ex-cond_1: pH: 6.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker AVANCE 800 MHz
Related Database Links:
| BMRB | 5662 5663 |
| PDB | |
| DBJ | BAA22995 |
| REF | NP_001179999 NP_999502 XP_004015712 XP_004284289 XP_004330765 |
| SP | O18734 |
| TPG | DAA19818 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts