BMRB Entry 5199
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5199
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Title: Assignment of the 1H and 15N resonances of the elicitor protein NIP1
Deposition date: 2001-11-02 Original release date: 2003-06-09
Authors: van, Klaas; 2, 5199; 3, 5199; 4, 5199; 5, 5199; 6, 5199; , 5199
Citation: van, Klaas; 2, 1; 3, 1; 4, 1; 5, 1; 6, 1; , 1. "Solution structure of the fungal plant disease resistance-triggering protein NIP1 shows a novel b sheet fold" . ., .-..
Assembly members:
Necrosis Inducing Protein 1, polymer, 60 residues, 6434 Da.
Natural source: Common Name: barley leaf scald fungus Taxonomy ID: 38038 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Rhynchosporium secalis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Necrosis Inducing Protein 1: DRCRYTLCCDGALKAVSACL
HESESCLVPGDCCRGKSRLT
LCSYGEGGNGFQCPTGYRQC
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 15N chemical shifts | 65 |
| 1H chemical shifts | 369 |
| coupling constants | 35 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NIP1 | 1 |
Entities:
Entity 1, NIP1 60 residues - 6434 Da.
| 1 | ASP | ARG | CYS | ARG | TYR | THR | LEU | CYS | CYS | ASP | |
| 2 | GLY | ALA | LEU | LYS | ALA | VAL | SER | ALA | CYS | LEU | |
| 3 | HIS | GLU | SER | GLU | SER | CYS | LEU | VAL | PRO | GLY | |
| 4 | ASP | CYS | CYS | ARG | GLY | LYS | SER | ARG | LEU | THR | |
| 5 | LEU | CYS | SER | TYR | GLY | GLU | GLY | GLY | ASN | GLY | |
| 6 | PHE | GLN | CYS | PRO | THR | GLY | TYR | ARG | GLN | CYS |
Samples:
sample-1: Necrosis Inducing Protein 1, [U-15N], 2.0 mM
conditions1: pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H COSY | not available | not available | not available |
| 2D 1H-1H NOESY | not available | not available | not available |
| 2D 1H-1H CLEAN-TOCSY | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 2D HMQCJ | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
| 3D 1H-15N-15N NOESY | not available | not available | not available |
| 3D 1H-1H-15N TOCSY | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
| 3D HNHB | not available | not available | not available |
Software:
NMRPipe -
XEASY -
NMR spectrometers:
- Bruker DMX 600 MHz
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts